扩张力作用下大鼠腭中缝组织成骨现象的连续观察  

Histological Change Sequence of Midpalatal Suture in Response to Maxillary Expansion in Growing Rats

作　　者：巫云霞[1] 王林[2] 张卫兵[2] 马俊青[2] 吴蓉宁[2] 张文健[2] 宋冬生[1] 

机构地区：[1]南京医科大学第一附属医院口腔科,江苏南京210029 [2]南京医科大学口腔医学院正畸科

出　　处：《口腔医学研究》2010年第3期309-311,共3页Journal of Oral Science Research

基　　金：国家自然科学基金资助项目(编号:30572068)

摘　　要：目的:观察扩张力作用下大鼠腭中缝牵张成骨的组织形态学变化及新骨形成情况,探讨扩弓后腭中缝组织的反应和新骨形成方式。方法:选用4周龄健康雄性wistar大鼠55只,随机分为实验组5组、对照组6组,采用两眼簧后牙扩弓器扩张大鼠上颌腭中缝组织,分别于0d、实验1、2、4、7、11d取材,标本常规进行石蜡切片,行常规苏木精-伊红染色、Masson三色染色、骨钙素(OCN)免疫组化。结果:扩张1d后即可见腭中缝明显增宽,并可见成骨细胞的分化和成纤维细胞的增殖;扩张2d和4d后可见软骨细胞带外侧有新骨沉积;固定3d后可见骨缝边缘内外侧均有新骨沉积;固定7d后,可见两侧陈旧的软骨细胞带内侧有大量类骨质形成,两侧骨缘呈指状嵌合。结论:扩张的腭中缝新骨改建主要发生在腭中缝骨缘,并由软骨内成骨的方式向膜内成骨方式进行转变。Objective：To study the sequence of histological changes and regularity of midpalatal suture osteogenesis during maxillary expansion.Methods：Fifty-five four-week-old male wistar rats were randomly divided into five experimental groups and six control groups（5 rats in each group）.In the experimental groups,rectangular appliances with two helical loops were set between the right and left upper molars to expand the midpalatal suture.The rats were sacrificed after 0 day,1 day,2,4,7 and 11 days after the activation respectively.Sections were cut perpendicular to the midpalatal suture and stained with hematoxylin and eosin for observation of celluar respons,and immunostained with anti-osteocalcin for evaluation of bone formation.The changes of collagen were observed through Masson staining.Results：One day after the force was applied,the pre-existing cartilaginous cell layers were laterally displaced.Stretched mesenchymal cells were arranged in parallel to the direction of expansion force and osteoblast differentiation was detected.Two days after expansion,a great amount of osteoblasts and fibroblasts proliferated.After the helical loops were fixed for three days,lots of proliferation cells were arranged in parallel with the margin of the suture.Another four days retention later,new bone formation was observed as digitation on the inner lateral side of the pre-existing cartilaginous cell layers.Conclusion：The bone formation mechanism can be altered from endochondral ossification in normal palatal suture to intramembranous osteogenesis on the inner lateral side of the pre-existing cartilaginous cell layers by the expansion force.

关 键 词：扩张力 腭中缝 缝牵张 骨改建 

分 类 号：R783.5[医药卫生—口腔医学]