传染性支气管炎病毒T株核衣壳蛋白(N)基因的克隆及部分序列分析  

CLONING AND SEQUENCING OF NUCLEOCAPSID PROTEIN GENE OF INFECTIOUS BRONCHITIS VIRUS

作　　者：陈丽君[1,2] 陈丽君[1,2] 蔡学忠[3] 王英[1,2] 王英 张平[1,2] 张平 张鸣[1,2] 张鸣 陈波[1,2] 陈波[1,2] 沈建英 沈建英[1,2] 王建荣[2,1] 王建荣[2,1] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106 [3]中国农业科学院上海家畜寄生虫病研究所,上海200232

出　　处：《上海农业学报》1999年第1期22-27,共6页Acta Agriculturae Shanghai

摘　　要：人工合成一双对应于ＩＢＶ－Ｂｅａｕｄｅｔｔｅ株Ｎ基因ＯＲＦ两侧序列的引物，应用ＲＴ－ＰＣＲ方法，获得肾型传染性支气管炎病毒Ａｕｓｔｒａｌｉａ－Ｔ株核衣壳蛋白基因，长度１．２３ｋｂ，利用引物设计中的ＥｃｏＲ　Ｉ位点将其克隆至载体ｐＳＫ（＋）中，对该基因进行限制性酶切分析，结果与已报道的相一致。通过Ｎ基因的Ｃ端部分序列分析及与Ｂｅａｕｄｅｔｔｅ株、Ｍ４１标准株和日本分离株相比较，结果表明该部分的序列有一定的差异，其同源率分别为８６．８％、８６．８％和８７．３％，推测的氨基酸同源率分别为８７．２％、８７．２％和８８．７％。Based on the reported sequence of the nucleocapsid protein gene, IBV Beaudette,two specific primers were synthesized. A 1.23 kb DNA fragment was amplicatedby RT-PCR using the viral RNA of IBV (strain Australia-T) as the template,and furthercloned into plasmid PSK (+). Restriction mapping revealed that the result was consistentwith the reported data. Comparison of sequence of N gene of Australia-T strain with otherIBV strains showed that the homologies were 86. 8%, 86. 8% and 87. 3% in nucleotide and87. 2 %, 87. 2%and 88. 7% in amino acid,respectively.

关 键 词：RT-PCR 核衣壳蛋白基因 序列分析 IBV 

分 类 号：S852.65[农业科学—基础兽医学]