快速检测柑桔黄龙病病原的研究  

Studies on the Rapid Detection of Citrus Huanglongbing Pathogen

作　　者：邓晓玲[1,2] 梁志慧[1,2] 唐伟文[1,2] 

机构地区：[1]华南农业大学资源环境学院 [2]华南农业大学食品科学系

出　　处：《华南农业大学学报》1999年第1期1-4,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金;广东省自然科学基金

摘　　要：应用ＰＣＲ技术对柑桔黄龙病病原ＤＮＡ进行体外扩增，可建立一套快速、准确、有效的检测该病原的方法研究结果表明：ＰＣＲ技术对柑桔黄龙病病原的检测具有很强的特异性，只有感染了黄龙病病原的样品，ＰＣＲ才呈阳性反应文章报道了应用ＰＣＲ技术能检测已带病但尚未显症状的柑桔黄龙病病株该检测技术可以对柑桔黄龙病进行早期诊断，及早地控制带病苗木的传播。The present study is aimed to develop an effective method for the detection of Liberobacter asiaticum that causes citrus Huanglongbing disease. It was shown that the DNA of the pathogen could be amplified by polymerase chain reaction (PCR) in vitro and the appearance of such DNA was significantly detected in the samples of infected citrus trees of the various cultivars. To use PCR for the detection of the infected trees without any symptom yet at the early stage of the infection. This technique can be effectively applied to screen disease free seedlings, establish disease free citrus nurseries, and control the spread of the diseased seedlings.

关 键 词：柑桔 黄龙病 PCR 检测 

分 类 号：S436.661.1[农业科学—农业昆虫与害虫防治]