中国禽肾病变IBV分离株S1基因的RT-PCR/RFLP特性  

Characterization by S1 Gene RT-PCR/RFLP of Nephropathogenic IBV Strains Isolated in China

作　　者：王林川[1] 张桂云[1] 黄勇[1] 李华 廖明[1] 

机构地区：[1]华南农业大学动物医学系

出　　处：《华南农业大学学报》1999年第1期32-36,共5页Journal of South China Agricultural University

基　　金：国家自然科学基金;广东省青年基金

摘　　要：对中国１３个省市分离到的２９个肾病变ＩＢＶ毒株作了Ｓ１基因的ＲＴ－ＰＣＲ／ＲＦＬＰ特性鉴定．用相同的一对引物，８个毒株———广东／０１／８３，河南／０４／９４，河南／０５／９４，内蒙／０１／９３，江苏／０１／９３，河南／０１／９３，天津／０２／９３，内蒙／０２／９３，经ＲＴ－ＰＣＲ扩增获得了包含有Ｓ１基因ｃＤＮＡ的１７３４ｂｐ的片段；河南／０６／９５的为一１０００ｂｐ左右的片段；其它２０个毒株没有结果．经ＲＦＬＰ分析，广东／０１／８３，河南／０４／９４，河南／０５／９４，内蒙／０１／９３归类于Ｍａｓｓａｃｈｕｓｅｔｓ血清型，江苏／０１／９３归于Ｇｒａｙ血清型，河南／０１／９３，天津／０２／９３，内蒙／０２／９３是新的变异株．研究结果表明，国内禽肾病变ＩＢ的致病毒株，在不同的地区有其特异的变异株。nephropathogenic IBV strains isolated from 13 provinces in China were characterized by S1 gene RT-PCR/RFLP. With the same pair of primers, eight IBV strains: Guangdong/01/83, Henan/04/94, Henan/05/94, Neimong/01/93, Jiangsu/01/93, Henan/01/93, Tianjing/02/93, Neimong/02/93, could amplify a sequence of 1734 bp that contains the S1 glycoprotein gene; the PCR product of strain Henan/06/95 was about 1 000 bp but the other 20 strains gave no result. By RFLP analysis,strains Guangdong/01/83,Henan/04/94,Henan/05/94, Neimong/01 /93 were classified as Massachusetts serotype, strain Jiangsu/01/93 was the same as Gray; strains Henan/01/93, Tianjing/02/93, Neimong/02/93 were new variants. Based on these observations, it is suggested that the causative agents of nephropathogenic IB in China were specific variants in different enzootic areas,and which also had a common serotype——Massachusetts IBV.

关 键 词：IBV S1基因 RFLP 肾病变 

分 类 号：S852.65[农业科学—基础兽医学] S855.3[农业科学—兽医学]