C型产气荚膜梭菌β毒素基因克隆与核苷酸序列分析  

Cloning and Nucleotide Sequencing of Beta Toxin Gene of Clostridium perfringens Type C

作　　者：许崇波[1,2,3] 王玉炯[1,2,3] 卫广森[1,2,3] 王卓 冯书章[1,2,3] 王文成 马成国[1,2,3] 李尚波 

机构地区：[1]中国人民解放军农牧大学军事兽医研究所 [2]宁夏农学院 [3]辽宁省益康生物制品厂

出　　处：《中国预防兽医学报》1999年第2期111-114,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：用ＰＣＲ技术，从含Ｃ型产气荚膜梭菌β毒素基因质粒ｐＢ１２中扩增出了０．９５Ｋｂ的β毒素基因，然后用限制性核酸内切酶ＢａｍＨＩ和ＥｃｏＲＩ对其进行双酶切处理，最后将其定向连接在事先经同样内切酶处理的载体ｐＥＴ－２８ｃ（＋）中的相应位点上，转化至受体菌ＢＬ２１（ＤＥ３）中。经ＢａｍＨＩ和ＥｃｏＲＩ双酶切分析和核苷酸序列分析，证明重组质粒ｐＸＥＴＢ２含有产气荚膜梭菌β毒素基因，确定了其全部的核苷酸序列。Beta toxin gene was amplificated from plasmid pB12 of Clostrdium perfringens type C by Polymerase Chain Reaction (PCR),PCR product was cleaved with restriction endonucleases BamH I and EcoR I,and inserted into the BamH I/EcoR I site of PET 28c(+) Vector.The recombinant plasmid pXETB2 was studied in detail by restriction endonuclease analysis and nucleotide seqencing.The 930bp fragment of beta toxin gene with positive reading frame was successfully cloned and sequenced.

关 键 词：产气荚膜梭菌 β毒素基因 基因克隆 核苷酸序列 

分 类 号：S852.61[农业科学—基础兽医学]