黄瓜绿斑驳花叶病毒检测技术的研究进展  

Review on the Detection Methods of Cucumber Green Mottle Mosaic Virus(CGMMV)

作　　者：罗梅[1] 王琳[2] 宾淑英[1] 林进添[1] 

机构地区：[1]仲恺农业工程学院植物保护系,广州510225 [2]广东省农业厅植物保护总站,广州510500

出　　处：《华中农业大学学报》2010年第3期392-396,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30671378)资助

摘　　要：黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)是我国的检疫性有害生物,在广西、辽宁、河北、山东、广东和北京等地均发现疫情,已严重威胁着西瓜、甜瓜、黄瓜等作物的生产,因此加强该病毒的检测极为重要。目前黄瓜绿斑驳花叶病毒病的检测主要有生物学检测、血清学检测、分子生物学检测及电镜显微观察等方法。笔者对有关黄瓜绿斑驳花叶病毒病的检测技术进行了综述,并分析了现有检测技术的优缺点和应解决的主要问题,探讨了该病毒检测技术今后发展的方向。The cucumber green mottle mosaic virus（CGMMV） is one of the quarantine viruses in China which can seriously affect the product of watermelon,melon and cucumber.It has been found in Guangxi,Liaoning,Hebei,Shandong,Guangdong Province and Beijing in China presently.Thus it is important to establish effective methods for the detection of CGMMV.Biology method,serology method,molecular biology method and electron microscopy method are widely used in the detection of CGMMV.This paper made a comprehensive study on the above mentioned methods and pointed out the advantages and disadvantages of these methods as well as the new development of the detection of this virus.

关 键 词：黄瓜绿班驳花叶病毒 检测 方法 

分 类 号：S482.39[农业科学—农药学]