木榄Na^+/H^+逆向转运蛋白基因的克隆  

Cloning of Na^+/H^+ Antiporter Gene from Bruguiera gymnorrhiza(L.) LAM

作　　者：郭庆水[1] 徐立新[1] 袁潜华[1] 于伟[1] 

机构地区：[1]海南大学农学院,海南海口570228

出　　处：《热带生物学报》2010年第2期105-109,共5页Journal of Tropical Biology

摘　　要：根据北滨藜、胡杨、毛白杨、番杏、盐爪爪、盐角草等已发表的逆向转运蛋白的保守区设计简并引物,通过RT-PCR和RACE技术,从木榄(Bruguiera gymnorrhiza)中克隆Na+/H+逆转运蛋白长度为2130bp的cDNA全长序列,开放阅读框1626bp,编码541个氨基酸和一个终止子,含10个跨膜区。蛋白序列同源分析表明它与蓖麻、胡杨、葡萄、百脉根等植物的液泡膜型Na+/H+逆向转运蛋白序列具有很高的同源性(80%以上),表明该序列确属Na+/H+逆转运蛋白家族的基因,在细胞中起到Na+区隔化作用。According to the homologous sequences from Atriplex gmelini, Populus euphratica, Tetragonia tetragonioides, Kalidium foliatum, Populus tomentosa and Salicornia europ, PCR primers were designed and RT-PCR and RACE were used to amplify NHX1 DNA fragment from the leaves of Bruguiera gymnorrhiza. The results indicated that the BgNHX1 contains 2 130 bp, including an open reading frame of 1 626 bp, which encodes a predicted polypeptide of 541 amino acids, and there are 12 predicted transmembrane domains. Base on the data that the sequence showed high homologies （ 〉 80% ） with Na^＋/H^＋ antiporter genes from Ricinus communis Populus euphratica, Citrus, paradisi, vitis vinifera, it was very possible that BgNHX1 was competent for Na^＋ compartmentation in cells.

关 键 词：木榄 BgNHX1 Na+/H+逆转运蛋白 同源 跨膜 

分 类 号：Q789[生物学—分子生物学]