不同盐浓度和光照强度对杜氏盐藻psbA基因表达的影响  

Effects of different salt concentration and light intensity on the psbA gene expression in Dunaliella salina

作　　者：吕文兵[1] 刘红涛[1] 薛乐勋[1] 

机构地区：[1]郑州大学生物工程系,郑州450001

出　　处：《华中师范大学学报（自然科学版）》2010年第2期288-292,共5页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金项目(30600006);科技部国际科技合作项目(2007DFA01240)

摘　　要：利用半定量RT-PCR和实时荧光定量PCR研究不同浓度的NaCl和不同的光照强度条件下杜氏盐藻psbA基因的表达差异.结果显示,杜氏盐藻在2.5 mol NaCl的培养基中,其psbAmRNA的表达达到最高,与其它各组不同NaCl浓度(1.5 mol,2.0 mol,3.0 mol和4.0 mol)相比,差异显著(P<0.05);高浓度的NaCl(4.0 mol)能明显抑制杜氏盐藻psbA基因的表达.此外,与暗培养相比,杜氏盐藻在3 000 lx和4 500 lx的条件下,psbA基因的表达量明显升高(P<0.05),其中4500 lx光强下psbA表达量达到峰值.但高光强(8 000 lx)与暗培养相比,psbA基因的表达量无差异(P>0.05).上述结果提示,高浓度的盐(4.0 mol)和高光强(8 000 lx)能明显抑制PsbA基因mRNA的表达;而适量浓度的盐和光照条件能促进PsbA基因mRNA的表达.To investigate changes of the psbA gene expression of Dunaliella salina （D. salina） under different concentrations of NaCl and light intensities. Semi-quantitative RT-PCR and Real-time quantitative PCR were employed to detect differential expression of psbA gene. The results revealed that psbA gene expression reached to the maximum under the concentration of 2.5 tool NaCl compared to the other concentrations NaCl （1.5 mol, 2.0 mol,3.0 mol and 4.0 mol）, and there were significant differences （P〈 0.05）. High concentrations NaCl （4.0 mol） markedly suppressed the psbA gene expression of D. salina. In addition, compared to 0 lx, relative expression of psbA gene was obviously enhanced under the condition of 3 000 lx and 4 500 lx light intensity （P〈 0.05）, in which psbA gene expression reached the maximum under the condition of 4 500 lx. However, there was no difference between 8 000 lx and 0 lx （P〉0.05）. The results above suggest that high concentration salt （4.0 mol） and high light intensity （8 000 lx） obviously inhibit the expression of psbA gene; while appropriate concentration salt and light intensity significantly improve the expression of psbA gene.

关 键 词：杜氏盐藻 PSBA基因 氯化钠 光照强度 

分 类 号：Q786[生物学—分子生物学]