光谱法研究痂囊腔菌素A与血红蛋白的相互作用  

Spectroscopic Studies on Interaction of Elsinochrome A With Hemoglobin

作　　者：马菲[1] 周林[1] 王薇[1] 冯玉英[1] 周家宏[1] 王兴和[2] 沈健[1] 

机构地区：[1]江苏省生物功能材料重点实验室,分析测试中心,南京师范大学,江苏南京210046 [2]南京师范大学物理科学与技术学院,江苏南京210046

出　　处：《南京师大学报（自然科学版）》2010年第2期46-49,53,共5页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金(20603018);江苏省教育厅自然科学基金(04KJD150110);江苏省光电技术重点实验室开放基金(KJC09)

摘　　要：利用紫外可见吸收光谱和荧光光谱系统地研究了痂囊腔菌素A(EA)与血红蛋白(Hb)之间的相互作用.实验结果表明,EA与Hb之间的相互作用影响Hb分子的构象,且该影响在避光与光照等两种情况下存在明显差异,避光时EA仅与Hb的氨基酸残基发生作用,而光照时EA与Hb的氨基酸残基和血红素辅基均发生作用.避光实验条件下的荧光猝灭常数、热力学参数等计算结果表明Hb荧光被猝灭方式为动态猝灭,EA对Hb之间的作用力为疏水作用力.同步荧光进一步研究了EA对Hb微环境的影响.The interaction between Elsinochrome A（EA） and hemoglobin（Hb） was investigated using UV-vis spectroscopy and fluorescence spectroscopy.The results suggested that the interaction modes were obviously different in the dark and the illuminated condition.In the dark,EA interacted with the surface amino acid of Hb only,but with the illumination,both amidic acid and the interior hemachrome of Hb interacted with EA.According to the values of the quenching constant and the thermodynamics parameters,the quenching mechanism was dynamic quenching procedure and the interaction between EA and Hb was mainly hydrophobic in nature.The changes in the micro-circumstance of amino acid of Hb were analyzed by synchronous fluorescence spectroscopy.

关 键 词：痂囊腔菌素A 血红蛋白 紫外可见吸收光谱 荧光光谱 同步荧光光谱 

分 类 号：O657.3[理学—分析化学]