溴氰菊酯抗感品系小菜蛾成虫差异表达蛋白的鉴定  

Identification of Differentially Expressed Proteins Between Deltamethrin Resistant and Susceptible Strains of Adult Plutella xylostella(L.)

作　　者：程滨[1] 张娜娜[2] 许勤[3] 杨小怡[2] 程罗根[2] 李忠英[4] 

机构地区：[1]淮南师范学院生命科学系,安徽淮南232001 [2]南京师范大学生命科学学院,江苏南京210046 [3]南京师范大学电气与自动化工程学院,江苏南京210042 [4]贵州省农业科学院植物保护研究所,贵州贵阳550006

出　　处：《南京师大学报（自然科学版）》2010年第2期87-90,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：安徽省教育厅重点项目(KJ2008A137);南京师范大学大学生实践创新计划项目

摘　　要：通过对小菜蛾Plutella xylostella(L.)抗溴氰菊酯品系和敏感品系成虫双向电泳图谱的比较,找到8个差异明显的点,并结合质谱分析技术和数据库信息检索最终鉴定出7个蛋白.其中包括2个代谢相关蛋白,2个调控蛋白,1个结构分子蛋白,其余2个蛋白质功能皆不明确.这些在抗性或敏感品系中差异表达蛋白质的发现,为进一步研究小菜蛾抗药性形成机制和抗性相关蛋白的寻找奠定了基础.Two-dimensional polyacrylamide gel electrophoresis was used to analyze protein changes in response to deltamethrin resistance in adult Plutella xylostella（L.）.After the analysis of the spectrum of gel electrophoresis,eight proteins were found different in expression between susceptible and deltamethrin-resistance strains.Combined with the application of MALDI-TOF-MS and similarity analysis to the database,seven proteins were identified successfully including two metabolism-related proteins,two regulatory proteins,one structural protein and two unknown protein.These differentially expressed proteins may be related to the insecticide resistance in Plutella xylostella（L.）,so the results lay a foundation for the further study of molecular mechanisms of the resistance.

关 键 词：小菜蛾 抗药性 双向电泳 质谱分析 溴氰菊酯 

分 类 号：S511[农业科学—作物学]