从HIF-2a基因筛选HiHiLo实施过程中预测血象指标变化的分子标记  

From the HIF-2a gene screening out molecular markers that predict the changing of hemogram indexes during the implementation of HiHiLo

作　　者：金溪[1] 胡扬[1] 罗冬梅[2] 李燕春[1] 衣龙彦[1] 

机构地区：[1]北京体育大学体育科学研究中心,北京100084 [2]北京体育大学运动解剖学教研室,北京100084

出　　处：《体育学刊》2010年第6期96-101,共6页Journal of Physical Education

基　　金：科技部奥运攻关课题(2006BAK12B01)

摘　　要：通过对HIF-2a/EPAS1基因多态性与HiHiLo训练前后血象指标进行关联分析,以期筛选预测HiHiLo实施过程中血象指标变化的分子标记。(1)对72名健康受试者进行4周的HiHiLo训练,在实验前后测量血象指标。(2)采用PCR-RFLP方法对HIF-2a/EPAS1基因多态位点进行解析。(3)对HIF-2a/EPAS1基因多态性与HiHiLo训练前后血象指标进行关联分析,探寻HiHiLo训练中评价血象效果的分子遗传学标记。结果发现:(1)WBC变化量在不同基因型之间差异存在显著性的趋势(P<0.10),M Retic#变化量差异存在显著性。(2)MCV、MCH变化量在rs6715787多态位点不同基因型之间差异存在显著性(P<0.01、P<0.05)。结果说明:(1)HIF-2a/EPAS1基因rs7598371多态位点与HiHiLo训练前后WBC和M Retic#相关联,可以作为HiHiLo训练中预测WBC和M Retic#变化的分子标记。(2)HIF-2a/EPAS1基因rs6715787多态位点与HiHiLo训练前后MCV和MCH相关联,可以作为HiHiLo训练中预测MCV和MCH变化的分子标记。The authors intended to screen out molecular markers that predict the changing of hemogram indexes during the implementation of HiHiLo by analyzing the correlation between the polymorphism of the HIF-2a/EPAS1 gene and hemogram indexes before and after HiHiLo training.1） the authors carried out 4-week HiHiLo training on 72 healthy testees,and measured hemogram indexes before and after the experiment;2） the authors dissected the polymorphic loci of the HIF-2a/EPAS1 gene by applying the PCR-RFLP method;3） the authors analyzed the correlation between the polymorphism of the HIF-2a/EPAS1 gene and hemogram indexes before and after HiHiLo training,and probed into molecular genetic markers for evaluating hemogram effects during HiHiLo training.The authors revealed the following findings： 1）there was a significant difference between WBC changes in different genotypes（P0.10）,and there was a significant difference between M Retic# changes;2）there was a significant differ-ence between MCV and MCH changes at the rs6715787 polymorphic locus in different genotypes（P0.01,P0.05）.The said findings indicated the followings： 1）the rs7598371 polymorphic locus of the HIF-2a/EPAS1 gene is correlative with WBC and M Retic# before and after HiHiLo training,can be used as a molecular marker to predict WBC and M Retic# changes during HiHiLo training;2）the rs6715787 polymorphic locus of the HIF-2a/EPAS1 gene is correlative with MCV and MCH before and after HiHiLo training,can be used as a molecular marker to predict MCV and MCH changes during HiHiLo training.

关 键 词：运动生物化学 高住高练低训 低氧诱导因子2基因 分子标记 血象指标 

分 类 号：G804.7[文化科学—运动人体科学]