RT-PCR一步法检测鸡传染性支气管炎病毒的研究  

Studies on the Detection of Avian Infectious Bronchitis Virus (IBV) by Access RT PCR

作　　者：尹燕博[1,2,3] 孙淑芳[1,2,3] 邹勇 徐兰芳[1,2,3] 马洪超 吴时友[1,2,3] 邰友华[1,2,3] 康顺文 龚振华[1,2,3] 郭福生 蒋正军 蔡丽娟 

机构地区：[1]农业部动物检疫所 [2]江西农业大学 [3]山东诸城市对外贸易集团公司

出　　处：《中国动物检疫》1999年第1期3-6,共4页China Animal Health Inspection

摘　　要：根据已发表的ＩＢＶＳ基因核苷酸序列，自行设计合成了一对跨幅约０．９ｋｂ的引物。该区段包括了Ｓ１基因Ｃ末端的４００ｂｐ和Ｓ２基因Ｎ末端的５００ｂｐ。用该引物对５株ＩＢＶ参考毒株Ｇｒａｙ、Ｍ４１、Ｈ１２０、Ｈ５２和ＭＡ５进行ＲＴ－ＰＣＲ一步法扩增均获得预期的ＰＣＲ产物，该引物的ＲＴ－ＰＣＲ灵敏度检测结果表明，可以检测出０．１１２ｐｇ／μＬ的模板。用该引物对３０个地方分离株进行检测，１８株呈阳性，与鸡胚传代及电镜观察结果一致。A pair of specific primers were designed according to the published sequences of IBV spike gene.The region between the primers were about 0.9kb , corresponding to sequences with 500bp of S1 gene C-terminus and 400bp of S2 gene N-terminus. The expected products of five IBVreference strains were obtained by Access RT PCR. The limit of IBV RNA detection by Access RT PCR assay was 0.112pg/μL of RNA sample.Eighteen strains of 30 isolat/es detected by the primer B were positive. These agreed with the results of the electronmicroscopy and embryo passage.

关 键 词：传染性支气管炎 病毒 IBV RT-PCR 检测 

分 类 号：S858.315.3[农业科学—临床兽医学] S854.43[农业科学—兽医学]