利用Dot- ELIS A检测Bt棉杀虫蛋白的研究  

Detection of Bt Toxin Protein in Transgenic Cotton by Dot-ELISA

作　　者：沈法富[1] 于元杰[1] 尹承佾[1] 刘风珍[1] 陈翠霞[1] 王留明 张军 

机构地区：[1]山东农业大学农学系,泰安271018 [2]山东省棉花研究中心

出　　处：《中国农业科学》1999年第1期15-19,共5页Scientia Agricultura Sinica

摘　　要：用苏云金芽孢杆菌ＨＤ－１和ＨＤ－６８晶体杀虫蛋白的碱溶产物作为抗原，制备相应的 抗血清，建立了检测 Ｂｔ杀虫蛋白的 Ｄｏｔ－ＥＬＩＳＡ，可检测 Ｂｔ杀虫蛋白的最低水平为 ８７．５ｎｇ／ｍｌ。 用Ｄｏｔ－ＥＬＩＳＡ对２个转基因抗虫棉和它们的亲本进行了检测，并与生物学测定的抗虫性进行 比较，结果基本一致。因此，对测定大批量转基因抗虫棉的抗虫性，Ｄｅｔ－ＥＬＩＳＡ是一个快速、敏 感、特异、有效的方法。The antigen and antiserum of crystal toxin protein of Bacillus thuringiensis srtains HD-1 and HD-68 which were soluble in alkali were prepared. A Dot-ELISA for Bt toxin protein had been developed. The detectable level of Bt toxin protein by Dot-ELISA was 87. 5ng/ml. Two Bt transgenic cotton and their parents were determined by Dot ELISA, and compared with bollworm resistance of these cotton in laboratory bioassay and field,The result was agreeable. It is concluded that the Dot-ELISA may be useful for rapid and effective detection in large samples for determining transgenic cotton resistance.

关 键 词：DOT-ELISA 转基因棉花 抗虫性 BT杀虫蛋白 

分 类 号：S562.01[农业科学—作物学]