短尾蝮蛇线粒体基因组全序列分析  

Complete Sequence Analysis of Short-Tailed Mamushi MT-genome

作　　者：陈念[1] 赖小平[1] 

机构地区：[1]广州中医药大学中药学院,中国广东广州510006

出　　处：《生命科学研究》2010年第3期189-194,共6页Life Science Research

基　　金：国家自然科学基金资助项目(K1060015)

摘　　要：参照近缘物种的线粒体基因序列设计并筛选得到8对引物,结合TA克隆和步移测序获得了全长17227bp的短尾蝮蛇线粒体基因组全序列.与多数蛇类线粒体基因组类似,其共编码包括13个蛋白、2个rRNA和22个tRNA在内的37个基因,另外还包含2个非编码的富含AT的控制区.基因间排列紧凑,多数基因间间隔极短甚至发生重叠.除nad1、cox1和nad3外,多数蛋白编码基因均以ATG作为起始密码子,终止密码子的使用则存在TAA、AGA、AGG和不完全的T4种情况.基于合并的19个tRNA基因序列组合数据采用NJ、MP和ME3种算法对21种蛇进行了初步的系统发育分析,结果表明,各主要分类单元之间的亲缘关系与前人基于形态学、线粒体12SrRNA和cytb基因序列研究的结论完全一致,这证实了基于合并的线粒体tRNA基因序列进行蛇类物种DNA分子系统学研究的可行性.The 17 227 bp complete mitochondrial genome of short-tailed Mamushi was amplified by eight pairs of primers that were designed on the basis of mtDNA sequences from other closely related snakes and sequenced by TA-cloning and primer-walking methods.The genome contain 37 genes,which include 13 protein-coding genes,2 ribosomal RNA genes and 22 transfer RNA genes（ranges from 55 to 73 bp）,as well as two putative non-coding control regions,a situation that is similar to that found in other snakes.Those genes are arranged compactly with no introns and few intergenic nucleotides.Most protein-coding genes begin with ATG as initiation codons except nad1,cox1 and nad3.Four types of termination codons,including TAA,AGA,AGG and incomplete T,were used by protein-coding genes.Based on the combined tRNA gene sequences,the phylogenetic tree was constructed with NJ,MP and ME methods and the phylogenetic relationships of 21 snakes were similar with most traditional conclusions based upon morphological characters and mitochondrial 12S rRNA and cyt b gene sequences.In conclusion,mitochondrial tRNA genes include informations that could be used for snake revolutionary reconstruction.

关 键 词：短尾蝮蛇 线粒体基因组 序列分析 系统发育 

分 类 号：Q343.17[生物学—遗传学]