原位鼻咽癌裸鼠模型血清蛋白质组学的研究  

Serum Proteomic Analysis of in situ NPC Nude Mouse Model

作　　者：吴丽莎[1] 蒋星军[2] 刘卫东[1] 周文[1] 冯湘玲[1] 李雪华[3] 祝斌[1] 王磊[1] 刘春梅[1] 姚开泰[1,4] 任彩萍[1] 

机构地区：[1]中南大学肿瘤研究所,中国湖南长沙410008 [2]中南大学湘雅医院神经外科,中国湖南长沙410008 [3]武警医学院病理学教研室,中国天津300162 [4]南方医科大学肿瘤研究所,中国广东广州510515

出　　处：《生命科学研究》2010年第3期250-256,共7页Life Science Research

基　　金：湖南省科技厅项目(2008FJ3186);973项目(2010CB833605);863项目(2007AA021811;2007AA021809);教育部新世纪优秀人才支持计划(NCET-10-0790)资助项目

摘　　要：利用我室建立的原位鼻咽癌裸鼠模型,建立了稳定性较好的鼻咽癌裸鼠血清蛋白质2-DE方法,并比较了原位鼻咽癌裸鼠血清与对照组裸鼠血清蛋白质2-DE图谱之间的差异.与正常裸鼠血清比较,原位鼻咽癌裸鼠模型组血清增加了4个蛋白质点.利用MALDI-TOF质谱技术对血清差异蛋白点进行鉴定,发现SAA-1前体(serumamyloidA-1 protein precursor)在鼻咽癌裸鼠模型组血清中明显高表达,为寻找鼻咽癌血清生物标志物提供了有利的线索.On the basis of in situ nasopharyngeal carcinoma（NPC）nude mouse model previously founded in our lab,stable 2-DE method for serum proteomic analysis of NPC nude mouse was established,and the 2DE profile of serum proteome of in situ NPC nude mouse was compared with that of control nude mouse.Compared to control nude mouse group,in situ NPC nude mouse group exhibited four more protein spots.By using MALDI-TOF mass spectrum technology,it was found that SAA-1 precursor was significantly overexpressed in the serum of in situ NPC nude mouse group.The results may provide a beneficial clue for exploring potential serum biomarks of NPC.

关 键 词：鼻咽癌(NPC) 血清蛋白质组学 双向凝胶电泳(2-DE) SAA-1 质谱分析 

分 类 号：R766.3[医药卫生—耳鼻咽喉科]