野生和栽培桔梗种质遗传多样性的SRAP研究  

SRAP Examination of Genetic Dicersity of the Wild Playtcodon grandiflourus and the Cultivation Playtcodon grandiflourus

作　　者：吴松权[1] 于亚彬[1] 严一字[1] 金东淳[1] 吴基日[1] 

机构地区：[1]延边大学农学院,吉林龙井133400

出　　处：《北方园艺》2010年第12期132-135,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(30660016)

摘　　要：采用SRAP分子标记技术，对8份野生和8份栽培桔梗种质资源进行遗传多样性研究，旨在为桔梗的遗传育种提供理论依据。结果表明：12对随机引物组合共扩增出109奈谱带，平均每对引物组合扩增出9．1条谱带，其中扩增出80条多态性谱带，平均每对引物组合扩增出6．7条多态性谱带，多态率为73．4％，显示出了较高的多态性。试验中SRAP数据的遗传相似系数范围为0．56～0．88，并以遗传相似系数0．56为标准，将16份桔梗种质资源分为野生桔梗和栽培桔梗二大类群。Sequence related amplified polymorphis（SRAP）molecular markers were used to detect the genetic diversity of 8 samples of the wild Playtcodon grandiflourus and 8 samples of the cultivation Playtcodon grandiflourus. The purpose of provide the basis for breedong of Playtcodon grandiflourus. The results showed that Twelve primer pairs produced 109 bands,veraged 9. 1 bands per primenr pair,and produced 80 polyrnorphic bands,veraged 6.7 polymorphic bands per primenr pair, showed a higher rate of polymorphic（73. 4%）. The SRAP data obtained genetic similarity coefficient range 0. 56~0. 88, the results showed that the 16 samples of Platycodon grandiflorum I3C. were clustered into the wild Playtcodon grandiflourus and the cultivation Playtcodon grandiflourus, two groups at genetic distance 0. 56.

关 键 词：桔梗 遗传多样性 SRAP标记 

分 类 号：S567.239[农业科学—中草药栽培]