培养基和生长调节剂对油松离体胚不定芽诱导的影响  

The Influence of Medias and Growth Regulators on Explants to Induce Adventitious Buds of Pinus tabulaeformis

作　　者：王永波[1] 唐德瑞[1] 马佩[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《北方园艺》2010年第12期149-151,共3页Northern Horticulture

基　　金：国家林业局"948"资助项目(980405)

摘　　要：以油松成熟胚为外植体，对影响不定芽诱导分化的各因子做进一步试验。结果表明：油松成熟胚不定芽诱导的最佳组合为1／2MS＋6-BA1．5mg／L＋NAA0．05mg／L，诱导率为59．3％。对其进行趋势面分析，6-BA浓度为1．7mg／L，NAA浓度为0．30～0．35mg／L时不定芽诱导率可达65％。诱导阶段平均增殖率为8，愈伤组织增殖培养后平均增殖率最大可达13。The mature embryo of Pinus tabulaeformis was used as explants. This paper made a study of the facts of multiple adventitious shoot differentiation from callus cultures. The results showed that the combination of the medium was 1/2MS＋6-BA 1. 5mg/L＋NAA 0. 2 mg/L,on which 59. 3% of explants was induced. Applying the method of trend surface model, when 6-BA 1. 7 mg/L and NAA 0. 3-0. 35. The highest induction rate of adventitious buds was 65%. Average rate of propagation was 8, after a generation of raise the highest average rate was 13.

关 键 词：油松 成熟胚 组织培养 不定芽 

分 类 号：S791.254[农业科学—林木遗传育种]