辣椒疫霉菌单个孢子囊快速分离及复壮技术研究  

Technology Research on the Single-sporangium Isolation and Rejuvenation of Phytophthora capsici

作　　者：柴贵贤[1] 巩振辉[1] 李大伟[1] 黄炜[1] 逯明辉[1] 陈儒钢[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《北方园艺》2010年第12期160-162,共3页Northern Horticulture

基　　金：国家自然科学基金资助项目(30571262;30771467);"十一五"国家科技支撑计划资助项目(2006BAD01A7);"13115"科技创新工程重大科技专项资助项目(2008ZDKG-03);教育部高校博士点基金资助项目(200807120007)

摘　　要：通过研究马铃薯葡萄糖琼脂培养基(PDA)和PDA含药选择性培养基(PDAPR)对辣椒疫霉菌单个孢子囊分离的影响,筛选出最佳进行单个孢子囊分离的培养基;利用一对抗、感品种作为寄主,通过常规抗病性鉴定技术,对陕西辣椒疫霉菌复壮前后的致病力进行了测定。结果表明:最佳进行辣椒疫霉菌单个孢子囊分离的培养基是PDA含药选择性培养基,其分离率高达95%,其中污染率只有15.8%;复壮前后菌株对感病品种的致病力有明显的差异,复壮后菌株的致病力比于复壮前提高27.2%,且与刚分离后菌株的致病力相当,说明通过复壮能够恢复辣椒疫霉菌菌株的致病力。By studying the effect of the potato dextrose agar（PDA）and PDA drug-containing selective culture medium （PDAPR）on the separation of individual zoosporangium to obtain the most effective culture for the separation of single Phytophthora capsici sporangium. We compared the pathogenicity of Phytophthora capsici before and after rejuvenation by using conventional techniques with one disease resistant host plant and a susceptible one. The results showed that the best culture for a single sporangium separation was the PDAPR, with a separation rate of 95%, where the pollution rate was only 15.8%. The pathogenicity of Phytophthora capsici after rejuvenation to susceptible plant was significant higher than that of before rejuvenationl. The virulence of Phytophthora capsici after rejuvenation increased was 27. 2% ,which shows that the strain pathogenicity can be restored back through rejuvenation.

关 键 词：辣椒疫霉菌 单个孢子囊 分离 复壮 致病力 

分 类 号：S641.3[农业科学—蔬菜学]