甲磺酸胺银杏内酯B对PAF诱导家兔血小板聚集和释放产物的影响  

The role of dimethylaminoethyl ginkgolide B mesylate on inhibiting platelet ag- gregation and release function

作　　者：刘洪[1] 潘苏华[2] 刘成鼎[2] 韦敏[2] 许惠琴[2] 

机构地区：[1]南京中医药大学图书馆,江苏南京210046 [2]南京中医药大学药学院,江苏南京210046

出　　处：《中成药》2010年第6期914-918,共5页Chinese Traditional Patent Medicine

基　　金：江苏省中医药局科研项目(H-024)

摘　　要：目的:探讨甲磺酸胺银杏内酯B抗血小板聚集和释放作用。方法:运用比浊法测定静脉给药5日后PAF诱导的家兔血小板聚集性,荧光分光光度法测定甲磺酸胺银杏内酯B对PAF诱导家兔血小板释放Ca2+的影响,放射免疫法测定其对PAF诱导家兔血小板中TXA2、PGI2含量的影响。结果:甲磺酸胺银杏内酯B1.95、3.90、7.80mg/kg对PAF诱导的家兔血小板聚集率有抑制作用,与空白对照组比较有显著性差异(P<0.01)。甲磺酸胺银杏内酯B3.90、7.80mg/kg显著抑制PAF诱导家兔血小板释放Ca2+(P<0.01)。甲磺酸胺银杏内酯B3个剂量组均抑制血小板释放TXB2,7.80mg/kg剂量组显著降低TXB2/6-keto-PGF1α比值(P<0.05)。结论:甲磺酸胺银杏内酯B具有抑制血小板聚集,减少其释放产物的作用。AIM： To investigate the inhibiting effect and mechanism of dimethylaminoethyl ginkgolide B mesylate on platelet aggregation and release function.METHODS： The effect of dimethylaminoethyl ginkgolide B mesylate on inhibiting PAF-induced platelet aggregation was measured by turbidimetry method through giving rabbits dimethylaminoethyl ginkgolide B mesylate at different final concentration via i.v.for 5 days.The release of Ca2 ＋ from PAF-induced platelet in rabbits was assayed with fluorospectrophotometry and the contents of TXA2 and PGI2 were measured by radio-immunity method.RESULTS： Three groups of dimethylaminoethyl ginkgolide B mesylate （1.95,3.90,7.80 mg/kg） had significant effect on inhibiting PAF-induced platelet aggregation in rabbits （compared to normal,P 0.01）.Two groups （3.90,7.80 mg/kg） had significant effect on inhibiting the release of Ca2 ＋ from PAF-induced platelet in rabbits （P 0.01）.Three groups had the effect on inhibiting the release of TXB2,and decreasing TXB2 /6-keto-PGF1α value.The group of 7.80 mg/kg had significant effect compared to normal （P 0.05）.CONCLUSION： Dimethylaminoethyl ginkgolide B mesylate could remarkably inhibit platelet aggregation in rabbits and reduce the release products.

关 键 词：甲磺酸胺银杏内酯B PAF 血小板聚集 Ca2+ TXA2 PGI2 家兔 

分 类 号：R283.6[医药卫生—中药学]