新疆阿魏水溶性抗溃疡有效成分的提取纯化工艺的研究  

Extraction and purification of water-soluble active ingredient of Ferula sinkiangensis

作　　者：徐晓琴[1] 滕亮[1] 戴秀勇 于富生 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830054 [2]新疆华圣元医药科技有限公司,新疆乌鲁木齐830011

出　　处：《中成药》2010年第6期931-936,共6页Chinese Traditional Patent Medicine

基　　金：新疆生产建设兵团博士基金项目(2008jc13)

摘　　要：目的:优选出新疆阿魏水溶性抗溃疡有效成分的提取工艺及纯化工艺。方法:以新疆阿魏水溶性提取物的出膏率和阿魏酸含量的综合评分为评价指标,采用均匀设计优选出新疆阿魏水溶性抗消化性溃疡有效成分的提取工艺和纯化工艺,并采用药效学试验对两种纯化方法进行比较、优选。结果:新疆阿魏水溶性抗溃疡有效成分的优化提取条件为:加水量为药材量8倍,提取2次,每次提取2.5h。高速离心纯化工艺条件为:药液浓度比1∶3,离心时间35min,离心转速4000r/min。ZTC1+l-Ⅱ天然澄清剂纯化工艺条件为:药液浓度比为1∶22,ZTC用量为22%,澄清时间为1h。结论:验证试验表明优化提取工艺稳定、合理、可行。药效试验结果表明提取液经过离心纯化后药效未出现显著降低,符合纯化要求,故确定采用高速离心法对药液进行纯化。AIM： To optimize the extraction and purification of water-soluble active ingredient of Ferula sinkiangensis.METHODS： First,the comprehensive scoring system was set up to reflect the change of the extraction rate and ferulic acid coutent.in combination with the uniform design.RESULTS： The optimized extracting process was as follows： adding 8 times water and extracting twice,every time lasting 2.5 h.The optimized centrifugation purificating process consisted of： concentration ratio （1 ∶ 3）,35 minutes centrifugal time,4 000 r/min rotation speed.The optimized ZTC1 ＋ l-Ⅱ clarificant purification process was as follows： concentration ratio was 1 ∶ 22,the content of pacificator was 22% ,purification time was 1 h.CONCLUSION： The optimized extraction is available,reasonable,and reproducible.The results of pharmacodynamic test show that centrifugation is suitable to the purification of extract liquid.

关 键 词：新疆阿魏 提取工艺 纯化工艺 均匀设计 

分 类 号：R284.2[医药卫生—中药学]