RP-HPLC同时测定坤泰胶囊中芍药苷、黄芩苷、盐酸巴马汀、盐酸小檗碱含量  

RP-HPLC determination of paeoniflorin,baicalin,palmatine hydrochloride and berberine hydrochloride in Kuntai Capsules

作　　者：杨燕飞 

机构地区：[1]江苏省镇江药品检验所,江苏镇江212003

出　　处：《中成药》2010年第6期958-960,共3页Chinese Traditional Patent Medicine

摘　　要：目的:采用高效液相色谱法测定坤泰胶囊(熟地黄、黄连、白芍、黄芩、阿胶、茯苓)中芍药苷、黄芩苷、盐酸巴马汀、盐酸小檗碱的含量。方法:Agilent ZORBAX SB-C18柱(4.6mm×250mm,5μm)为固定相,以乙腈-0.4%磷酸溶液洗脱,流速为1.0mL/min,检测波长为230nm。结果:芍药苷、黄芩苷、盐酸巴马汀、盐酸小檗碱的线性范围分别为0.01658～0.3316μg(r=0.9999),0.05980～1.1960μg(r=0.9999),0.01291～0.2582μg(r=0.9999),0.04198～0.8396μg(r=0.9999)。加样回收率分别为98.91%(RSD为1.29%),99.20%(RSD为0.92%),97.86%(RSD为0.89%),98.63%(RSD为0.62%)。结论:该方法简便易行,重复性好,可用于对坤泰胶囊的质量控制。AIM： To develop an HPLC method for analyzing the contents of paeoniflorin,baicalin,palmatine hydrochloride and berberine hydrochloride in Kuntai Capsules（Praeparata Radix Rehmannial,Rhizoma Coptidis,Radix Paeoniae,Radix Scutellariae,Colla Asini corii,Poria）.METHODS： Four compounds were determirned with an Agilent ZORBAX SB C18 column（4.6 mm × 250 mm,5 μm）.Acetonitrile-0.4% phosphoric acid was used as the mobile phase at a flow rate of 1.0 mL/min.The mobile phase was in linear gradient elution.The detection wavelength was 230 nm.RESULTS： The linear ranges were within 0.016 58-0.331 6 μg（r = 0.999 9） for paeoniflorin,0.059 80-1.196 0 μg（r = 0.999 9）for baicalin,0.012 91-0.258 2 μg（r = 0.999 9）for palmatine hydrochloride,0.041 98-0.839 6 μg（r = 0.999 9） for berberine hydrochloride.The average recoveries （n = 6） were 98.91% （RSD = 1.29% ）,99.20% （RSD = 0.92% ）,97.86% （RSD = 0.89% ）,98.63% （RSD = 0.62% ）,respectively.CONCLUSION： The method is simple,reliable,sensitive and can be applied to having controls over the preparation.

关 键 词：坤泰胶囊 芍药苷 黄芩苷 盐酸巴马汀 盐酸小檗碱 

分 类 号：R927.2[医药卫生—药学]