HPLC同时测定感冒软胶囊中盐酸麻黄碱、盐酸伪麻黄碱及葛根素、黄芩苷含量  

HPLC determination of four active components in Ganmao Soft Capsule

作　　者：韩杰[1] 李东辉[1] 王鑫[1] 

机构地区：[1]北京市海淀区药品检验所,北京100083

出　　处：《中成药》2010年第6期963-967,共5页Chinese Traditional Patent Medicine

摘　　要：目的:用高效液相色谱法测定感冒软胶囊(麻黄、葛根、黄芩、桂枝、当归、白芷等)中盐酸麻黄碱、盐酸伪麻黄碱2种生物碱异构体成分的含量和葛根素、黄芩苷2种黄酮类成分的含量,为制定该制剂质量标准中含量测定方法及限度提供依据。方法:测定盐酸麻黄碱与盐酸伪麻黄碱用色谱柱为kromasilTMC18分析柱(4.6mm×250mm,5μm);流动相:乙腈-0.02mol/L磷酸二氢钾(磷酸调节pH值为2.70)(2∶98,含0.1%三乙胺);流速:1mL/min;检测波长:207nm;柱温:40℃;进样量:10μL。测定葛根素与黄芩苷用色谱柱为kromasilTMC18分析柱(4.6mm×150mm,5μm);流动相:乙腈-水(11∶89);流速:1mL/min;检测波长:250nm;柱温:30℃;进样量:10μL。结果:该方法回收率盐酸麻黄碱:99.96%(RSD=1.25%),盐酸伪麻黄碱:98.41%(RSD=1.46%),葛根素:98.67%(RSD=0.91%),黄芩苷:99.30%(RSD=0.88%)。结论:结果表明该方法准确可靠,重现性好,结果稳定。AIM： To establish the methods for determining ephedrina hydrochloridum,pseudoephedrine hydrochloride,puerarin,baicalin in Ganmao Soft Capsule （Herba Ephedrae,Radix Puerariae lobatae,Radix Scutellariae,Ramulus Cinnamomi,Radix Angelicae sinensis,Radix Angelicae dahuricae,etc.） METHODS： Part 1（determination of ephedrina hydrochloridum and pseudoephedrine hydrochloride）：The HPLC was carried out on kromasilTMC18 column（4.6 mm × 250 mm,5 μm） with acetonitrile-0.02 mol/L phosphoric acid solution（pH2.7）-triethylamine（2 ∶ 98 ∶ 0.1）as mobile phase,gradient elution.The detection wavelength was set at 207 nm.Part 2（determination of pueranin and baicalin）：The HPLC was carried out on kromasilTMC18 column（4.6 mm × 150 mm,5 μm）with acetonitrile-water（11 ∶ 89）as mobile phase,gradient elution.The detection wavelength was set at 250 nm.RESULTS： The average recoveries were 99.96% with RSD 1.25% ,98.41% with RSD 1.46% ,98.67% with RSD 0.91% ,99.30% with RSD 0.88% ,respectively.CONCLUSION： The assay results demonstrate that the methods are simple,accurate and reproducible for quantification of four active components in Ganmao Soft Capsule.

关 键 词：HPLC 感冒软胶囊 盐酸麻黄碱 盐酸伪麻黄碱 葛根素 黄芩苷 

分 类 号：R927.2[医药卫生—药学]