芫荽中总黄酮的提取及其对羟自由基的清除作用  

Extracting of total flavanone from coriander and its effects on scavenging of hydroxyl radicals

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作  者:唐玉莲[1] 黎海妮[1] 刘海花[1] 黄锁义[2] 

机构地区:[1]右江民族医学院医学检验学院,广西百色533000 [2]右江民族医学院药学系,广西百色533000

出  处:《国外医学(医学地理分册)》2010年第2期96-98,共3页Foreign Medical Sciences:Section of Medgeography

基  金:广西十五规划重点项目(桂教科学[2008]2号)

摘  要:目的为充分利用芫荽植物资源,探讨芫荽中总黄酮的提取、鉴别及对羟自由基清除作用。方法采用超声波乙醇浸提法从芫荽中提取黄酮类物质,对所提取的黄酮类物质进行验证,并用分光光度法测定含量,用芫荽中总黄酮对羟自由基清除作用进行试验。结果测得样品中总黄酮的含量C=0.42 g/L,回收率为102.3%,其纯度和产率均较高。结论该方法采用全物理过程,无任何污染,是提取芫荽中黄酮类物质的有效途径。芫荽中总黄酮提取液对Fenton体系产生的·OH自由基有很好的清除作用。Objective In order to make use of the resources of coriander, avoid wasting and approach the extraction of total flavanone of coriander and its effects on scavenging of hydroxyl radicals. Methods The flavonids were extracted by ethanol as the solvent from coriander with ultrasonic wave and ethanol extraction. Using spectrophotometry to extract and check the flavanone of coriander. The effect of extracts on coriander scavenging of hydroxyl radicals was studied. Results By this method getting the density of the total flavanone of coriander is C=0. 42 g/L and the rate of recovery is 102.3%. Conclusion This text provides the extraction and purifying methods to get the outcome and the purity of the flavanone are all very high . This method is a purely physical process and has not any pollution. It is an ideal way to extract the flavanone of coriander. Total flavanone from coriander have effects on scavenging of hydroxyl radicals.

关 键 词:超声波提取 芫荽 总黄酮 鉴别 自由基 清除 

分 类 号:O624.42[理学—有机化学]

 

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