HPLC测定五灵脂中原儿茶酸含量  

Determination of Protocatechuic Acid in Trogopterus xanthippes Milne-edwards by High Performance Liquid Chromatography

作　　者：朱翔宇[1] 杨莲菊[2] 李春[2] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2010年第6期71-73,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30873382)

摘　　要：目的:建立了五灵脂中活性成分原儿茶酸的HPLC含量测定方法。方法:采用Hypersil GOLD C18色谱柱;流动相为甲醇-1%冰醋酸水(5︰95);流速为1 mL.min-1;检测波长260 nm。结果:原儿茶酸的线性范围为0.019 9～0.099 5μg,线性相关系数为0.999 9。其精密度、稳定性、重现性和回收率均在允许限定范围内。结论:该方法简便、可靠,为五灵脂药材的质量控制提供了科学依据。Objective：A method was developed for the quantitative determination of protocatechuic acid in Trogopterus xanthippes Milne-Edwards by HPLC.Method： The analyst was determined by HPLC with a Hypersil GOLD C18 column and a mobile phase of menthol-1% acetic acid-water（5∶ 95）,at a flow rate of 1 mL·min^-1.The UV detection wave length was 260 nm.Result： The assay exhibited a good linearity in the concentration range from 0.019 9 to 0.099 5 μg with a correlation coefficient of 0.999 9.The analyst ’s precision,stabilization,reappearance and recovery were all in limited range.Conclusion：This method was reliable and could be used for the quality control of Trogopterus xanthippes Milne-Edwards.

关 键 词：五灵脂 原儿茶酸 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]