胃肠舒对糖尿病大鼠胃窦平滑肌细胞线粒体的影响  

Effect of Weichangshu on Mitochondrial of Stomach Smooth Muscle Cells in Rats with Diabetic Mellitus

作　　者：宋晓冬[1] 张瑾锦[1] 刘文波[1] 王秀文[1] 

机构地区：[1]滨州医学院实验中心,山东烟台264003

出　　处：《中国实验方剂学杂志》2010年第6期129-133,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30672680;30873263);烟台市科技攻关项目(2008162);大学生科技创新计划(BY2007DKCX10)

摘　　要：目的:研究中药胃肠舒对糖尿病(diabetes mellitus,DM)大鼠胃窦平滑肌细胞线粒体形态、结构、功能和蛋白的影响,探讨胃肠舒促胃肠运动的线粒体动能调控机制。方法:选择体重150～170 g的SD大鼠随机分为4组:正常对照组、DM组、DM+胃肠舒Ⅰ组(750 mg.kg-1)、DM+胃肠舒Ⅱ组(1 500 mg.kg-1)。透射电子显微镜检测线粒体形态、结构的变化,流式细胞术检测线粒体跨膜电位的变化,荧光测定法检测线粒体呼吸链的变化,Western Blot检测线粒体蛋白的变化。结果:胃肠舒能改善DM大鼠胃窦平滑肌细胞线粒体的形态、结构,增强呼吸链功能,线粒体跨膜电位和ATP含量增高,细胞色素C氧化酶(cytochromeC oxidase,COX)活性增强,活性氧自由基(ROS)释放减少,凋亡蛋白家族Bcl-2表达上调,Bax表达下调。结论:胃肠舒促胃肠运动可能与改善平滑肌的线粒体动能系统有关。Objective：To study the mechanism of the Chinese medicine Weichangshu on mitochondrial kinetic energy of stomach in diabetic mellitus（DM） rats by measuring the changes in morphology,structure,function and protein of mitochondria.Method：Standard Sprague Dawley rats（150-170 g） were randomly divided into 4 groups,including a control group,DM model group,DM ＋ WeichangshuⅠgroup（750 mg·kg^-1）,DM ＋ Weichangshu Ⅱ group（1 500 mg·kg^-1）.The mitochondrial morphology and structure were detected using transmission electron microscopy.The changes in mitochondrial transmembrane potential,respiratory chain and mitochondrial proteins were detected using flow cytometry,fluorescence labeling and Western blot respectively.Result：Weichangshu could ameliorate the mitochondrial morphology,structure and respiratory chain of stomach in diabetic mellitus rats.Mitochondrial transmembrane potential,ATP level and activity of cytochrome C oxidase were increased.The release of ROS was decreased.The expression of Bcl-2 was up-regulated and the expression of bax was down-regulated.Conclusion：Weichangshu could promote the gastrointestinal motility,which might be related with the regulation of mitochondrial kinetic energy.

关 键 词：胃肠舒 胃肠运动 线粒体 跨膜电位 呼吸链 

分 类 号：R285.5[医药卫生—中药学]