肺瘤平膏及其拆方含药血清对人外周血树突状细胞迁移功能影响及机制研究  

Investigation on Migration Ability and Mechanism of Dendritic Cells by Feiliuping Extract and its Modified Formula

作　　者：周雍明[1] 朴炳奎[1] 郑红刚[1] 熊露[1] 赵保胜[2] 

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2010年第6期152-155,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金(30371821)

摘　　要：目的:探讨中药肺瘤平膏及其拆方对树突状细胞(dendritic cell,DC)迁移功能的影响及分子机制。方法:采用transwell小室法测定DC的迁移能力;realtime-PCR法测定DC表面CCR7,CXCR4等趋化因子受体mRNA的表达。结果:解毒中药有抑制DC迁移的趋势,而肺瘤平膏、益气药物、活血药物则可促进DC迁移;在不同趋化因子作用下,药物对DC迁移的影响不同;不同药物作用下,DC表面趋化因子受体表达不同,益气组CCR7表达最高,活血组、肺瘤平组次之,解毒组最低,益气组、活血组与解毒组比较,P<0.05;CXCR4在益气组、肺瘤平组表达最高,活血组次之,解毒组最低,肺瘤平组、益气组、解毒组与对照组比较,P<0.01,解毒组与其他各组比较,P<0.01。结论:肺瘤平膏及益气组分、活血组分对DC的迁移有促进作用,解毒组分则明显抑制DC的迁移,不同中药作用下DC趋化能力的改变可能与趋化因子受体的表达有关。Objective：To observe the migration ability and mechanism of dendritic cells（DC） by Feiliuping extract and its modified formula.Method：The migration ability of DCs were determinated by transwell bordens,the expression of CCR7,CXCR4 on surface of DC were detected by RT-PCR.Result：Different TCM has different influence on migration ability of DCs.And ability was different with different chemotactic factor.The expression of CCR7,CXCR4 on surface of DC were different with different TCM,CCR7 in Yiqi group was the highest,while Huoxue group,FLP group lower,Jiedu group is the lowest.Comparing with Yiqi group,Huoxue group Jiedu group（P 〈0.05）.CXCR4 in Yiqi Group FLP group was highest,Huoxue group is lower,Jiedu group is the lowest.Comparing with blank serum group and FLP group,Yiqi group,Jiedu group（P 〈0.01）.Conclusion：Yiqi herbs strengthens migration ability of DCs,while Jiedu herbs inhibits.The difference was related to the expression chemotactic factor receptor on DCs surface.

关 键 词：肺瘤平膏 树突状细胞 迁移 

分 类 号：R285.5[医药卫生—中药学]