蒲黄-五灵脂药对在少腹逐瘀汤活血化瘀效应中的贡献  

Effects of Promoting Blood Circulation to Remove Blood Stasis of Puhuang-Wulingzhi Drug Pair in Shaofu Zhuyu Decoction

作　　者：周卫[1] 宿树兰[1] 刘培[1] 华永庆[1] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省方剂研究重点实验室,南京210046

出　　处：《中国实验方剂学杂志》2010年第6期179-183,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省中医药管理局资助项目(LB09016);国家自然科学基金资助项目(30973885);江苏省"青蓝工程"科技创新团队建设项目资助(2006-C-20);南京中医药大学青年自然科学基金资助项目(09XZR15)

摘　　要：目的:评价少腹逐瘀汤、少腹逐瘀汤减蒲黄-五灵脂及蒲黄-五灵脂药对水提物对大鼠急性血瘀模型血液流变性的影响及体外对家兔血小板聚集及凝血酶时间的影响。方法:采用大鼠急性血瘀模型评价少腹逐瘀汤、少腹逐瘀汤减蒲黄-五灵脂及蒲黄-五灵脂药对水提物对血液流变性的影响;采用体外实验观察不同提取物对二磷酸腺苷(ADP)诱导的血小板聚集作用及凝血酶时间(TT)法抗凝血作用的影响。结果:大鼠急性血瘀模型研究结果表明:少腹逐瘀汤能够明显改善急性血瘀SD大鼠全血黏度(P<0.05)、血沉(P<0.05);且可以延长TT、凝血酶原时间(PT)和部分活化凝血活酶时间(APTT);而蒲黄-五灵脂药对在降低全血黏度、改善血沉及体外延长家兔血浆凝血时间方面均优于全方减去蒲黄-五灵脂提取物。结论:少腹逐瘀汤对大鼠急性血瘀模型的血液流变性及体外对家兔血小板聚集及凝血酶时间的影响均有显著作用,且蒲黄五灵脂药对对于全方效应起到主要作用,提示蒲黄-五灵脂药对与全方减去蒲黄-五灵脂药味配伍可能存在一定的增效作用,为揭示该药对对全方活血化瘀效应的贡献提供了一定科学数据。Objective：To evaluate the effects of Shaofu Zhuyu decoction,prescription minus Puhuang-Wulingzhi drug pair and Puhuang-Wulingzhi drug pair on hemorheological parameters in rat mode of acute blood stasis,and the influence on platelet aggregation and clotting time in vitro.Method：Rat mode of acute blood stasis was used to evaluate the Changes in hemorheology,the function of blood coagulation and ovarian.The platelet aggregation was tested with Born′s method and thrombin time（TT） method in vitro was established to determine the clotting time.Result：The results stated that Shaofu Zhuyu decoction showed significant improvements in the hemorheological indexes of rats in the model of blood stasis and regulation for the secret function of rat ovarian.Meanwhile,the result of vitro assay showed that Shaofu Zhuyu Decoction had significantly inhibitory effects on the platelet aggregation and prolonged clotting time.The effects of Puhuang-Wulingzhi drug pairare superior to the group of prescription minus Puhuang-Wulingzhi drug pair on reducing whole blood viscosity,plasma viscosity and extending clotting time in vitro.Conclusion： Shaofu Zhuyu decoction can improve the indexes of hemorheology in rat model of blood stasis,and showed significant inhibitory effect on platelet aggregation and clotting time.Furthermore,Puhuang-Wulingzhi drug pair plays the major role in the biological effect for the whole prescription.

关 键 词：蒲黄 五灵脂 药对 少腹逐瘀汤 活血化瘀 

分 类 号：R285.5[医药卫生—中药学]