蜜炙甘草饮片HPLC指纹图谱研究  

HPLC fingerprint spectrum of honey-fried Radix Glycyrrhizae

作　　者：周倩[1] 吕佳[2] 李贵海[1] 石典花[1] 戴衍朋[1] 孙立立[1] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东师范大学,山东济南250014

出　　处：《中国中药杂志》2010年第12期1547-1550,共4页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2006BAI09B06-08)

摘　　要：目的:建立蜜炙甘草饮片高效液相指纹图谱。方法:采用高效液相色谱法,选用Hyperclone ODS C18柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸作为流动相梯度洗脱。结果:建立了蜜炙甘草饮片的HPLC指纹图谱,方法学考察结果符合指纹图谱技术要求。结论:该方法稳定、准确、可靠,为蜜炙甘草饮片质控标准的制定提供了科学依据。Objective： To establish the HPLC fingerprint of the pieces of honey-fried Radix Glycyrrhizae.Method： Using the reverse-performance liquid chromatography,method was performed on a Hyperclone ODS C18 column（4.6 mm×250 mm,5 μm） and acetonitrile-0.1% phosphoric acid was selected as mobile phase gradient elution were adopted.Result： Established HPLC fingerprint of Radix et Rhizoma glycyrrhizae pieces were established,and the results of methodological study met the technical requirements for fingerprinting.Conclusion： The HPLC method is stable,accurate,and reliable to provide a scientific basis of quality control standard for the honey-fried Radix et Rhizoma glycyrrhizae.

关 键 词：蜜炙甘草饮片 HPLC 指纹图谱 

分 类 号：R284[医药卫生—中药学]