双黄连冻干粉透过妊娠大鼠胎盘屏障的药物成分研究  

Ingredients of Shuanghuanglian injection powder permeation through placental barrier of rat in pregnancy

作　　者：宋殿荣[1] 郭洁[1] 王跃飞[2] 潘桂湘[2] 李沛霖[1] 张崴[1] 宋红运[1] 

机构地区：[1]天津中医药大学第二附属医院妇科,天津300150 [2]天津中医药大学中医药研究院省部共建教育部方剂重点实验室,天津300193

出　　处：《中国中药杂志》2010年第12期1626-1629,共4页China Journal of Chinese Materia Medica

基　　金：国家科技部中医药行业专项(200707011)

摘　　要：目的:对不同孕期妊娠大鼠给予双黄连冻干粉,采用高效液相色谱技术,研究双黄连冻干粉透过胎盘屏障的药物成分。方法:早、中、晚孕期W istar大鼠尾静脉注射双黄连冻干粉,给药5 d,末次给药后12 h,取血及胚胎组织,采用高效液相色谱技术,分析生物样品中所含药物成分。结果:早、中、晚孕期大鼠血浆中均含有黄芩苷、木犀草素、汉黄芩苷;各孕期胚胎组织中均含有黄芩苷,早期胚胎组织中还含有汉黄芩苷。结论:黄芩苷是双黄连冻干粉透过胎盘屏障的主要药物成分,对其胚胎毒性的研究将成为评价双黄连冻干粉妊娠期应用安全性的关键。Objective： Use HPLC to study the permeation of ingredients of Shuanghuanglian injection powder（SHL） through placental barriers of rats at different stages of pregnancy.Method： The pregnant rats were administered SHL for 5 d through caudalis vena at different stages of pregnancy.Plasma and embryonic tissues were obtained 12 h after the final administration of SHL.The componds in biological specimen were identified by HPLC.Result： Baicalin,luteolin and wogonoside were the main compounds in plasma.Wogonoside retained in first trimester embryonic tissues,and baicalin retained in the embryonic tissues of different pregnant stages.Conclusion： Baicalin is the main compound of SHL through placental barriers of rats.Embryotoxicity of baicalin should be considered as the key point to evaluate the safety of SHL.

关 键 词：双黄连冻干粉 黄芩苷 胎盘屏障 液相色谱 

分 类 号：R285.5[医药卫生—中药学]