松果菊苷对大鼠急性肺损伤模型早期干预作用研究  

Effect of early intervention of echinacoside on acute lung injury in rats

作　　者：张雁林[1] 艾铁民[2] 徐庆栋 邢俊杰[1] 赵金垣[1] 

机构地区：[1]北京大学第三医院职业病研究中心,北京100083 [2]北京大学药学院,100083 [3]山东省日照市五莲中医院,262300

出　　处：《中国职业医学》2010年第3期187-189,193,共4页China Occupational Medicine

基　　金：国家自然科学基金资助项目(30571551)

摘　　要：目的探索大鼠急性肺损伤/急性呼吸窘迫综合征(ALI/ARDS)的自由基变化及早期应用自由基清除剂松果菊苷(ECH)的防护作用。方法本研究分设4个研究组:对照组、ECH组、油酸(OA)组、OA-ECH组,分别在5个时间点采取标本检测动脉血气、肺组织活性氧(ROS)信号、肺湿/干重比值和肺病理。结果在各组中ALI的程度与自由基的相对浓度具有一致性,自由基的改变(30min)明显早于肺组织病理学(2h)改变。在注射OA后2h,OA组肺自由基达130～140高斯(Gauss),氧分压降至45～55mmHg;而OA-ECH组自由基是70～80Gauss,氧分压在65～75mmHg。肺湿/干重比值和病理学评分在2组差异也有统计学意义(P<0.01)。ECH通过减少ROS的生成,可明显减轻ALI的病理改变和低氧血症。结论 ROS是ALI发病过程的启动因子,早期应用抗氧化剂ECH可在一定程度上改善ALI的预后。Objective To study the changes of free radicals in rats with acute lung injury/acute respiratory distress syndrome （ALI/ARDS） and the protecting effect of echinacoside （ECH） against ALI/ARDS in rats.Methods The rats were divided into 4 groups：the control,ECH,oleic acid （OA） and OA-ECH.The arterial blood gases,free radical signal in lung tissues,lung wet/dry weight ratio and pulmonary histopathology were detected at 5 different time points.Results The degrees of ALI were corresponding with the relative concentrations of free radicals in all groups.The change of free radicals （30 minutes） was earlier than that of pathological characters （2 hours）.2 hours after OA injection,the free radicals in OA group reached to 130~140 Gauss,PaO2 decreased to 45~55 mmHg.While in ECH protection group,the free radicals reached to 70~80 Gauss,PaO2 decreased to 65~75 mmHg.The lung wet/dry weight ratio and pathological score were also significantly different between the two groups（P0.01）.ECH could decrease the signal of free radicals in ALI rats and protect against the injury.Conclusion It was strongly suggested that the reactive oxygen species （ROS） is the trigger in the pathogenesis of ALI.Antioxidants can improve the prognosis of ALI by scavenging the excess free radicals.

关 键 词：急性肺损伤 活性氧 松果菊苷 电子自旋共振 油酸 

分 类 号：R135.1[医药卫生—劳动卫生] R135.2[医药卫生—公共卫生与预防医学]