丙烯酸甲酯对小鼠肺组织中肿瘤坏死因子-α及白细胞介素-6水平的影响  

Effect of methyl acrylate on levels of TNF-α,IL-6 in mice lung tissue

作　　者：王立辉[1] 冯三畏 王振全[1] 连素琴[1] 严俊[1] 赵春霞[1] 张晴晴[1] 

机构地区：[1]兰州大学公共卫生学院劳动卫生与环境卫生研究所,甘肃兰州730000 [2]中国石油兰州公司总医院职业病科,730060

出　　处：《中国职业医学》2010年第3期227-229,232,共4页China Occupational Medicine

摘　　要：目的探讨丙烯酸甲酯(MA)对小鼠肺组织的损伤作用机制。方法昆明种小鼠40只,体质量18～22g,雌雄各半,随机分为对照组和MA吸入染毒280、560、1120mg/m3组,置于静式染毒柜中,每天吸入染毒2h,连续染毒30d,末次染毒后禁食24h处死,称量各组小鼠的脏器系数,观察病理形态学改变,采用酶标仪检测肺组织中的肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平的变化。结果与对照组比较,除染毒280mg/m3组TNF-α水平略有降低外,其余各染毒组TNF-α和IL-6水平都有不同程度的升高(P<0.05);肺心指数和肺脏湿干比与对照组比较差异无统计学意义(P>0.05);光镜观察显示:MA染毒各组小鼠肺组织均有炎性细胞浸润、充血,1120mg/m3组肺组织有水肿出现。结论 MA可导致小鼠肺组织中TNF-α、IL-6水平的升高,引起小鼠肺组织的炎性损伤。Objective To explore the effect of methyl acrylate （MA） on pneumonocyte injury in mice and to elucidate an immunotoxic mechanism of MA.Methods 40 Kunming mice were randomly divided into 4 groups as follows：normal control group,280 mg/m3,560 mg/m3 and 1 120 mg/m3 MA inhalation group.Mice exposed to different concentrations of MA through static inhalation,2 hours a day for 30 days.24 hours after the last exposure,the mice were executed and the relative weights of lung and heart were calculated.The upper lobe of right lung was taken for routine pathological examination.The contents of IL-6 and TNF-α were measured with enzyme-linked immunosorbent assay （ELISA）.Results Compared with the control group,the levels of TNF-α and IL-6 in lung tissue significantly increased except for TNF-α at 280 mg/m3 group （P0.05）.Cardiopulmonary index and lung wet/dry weight ratio were not caused statistically increase（P0.05） in all MA inhalation groups.Under the optical microscopy,lung tissue in each concentration group was characterized by an infiltrate of inflammatory cells and hyperaemia.Oedema was observed in the group of 1 120mg/m3.Conclusion Pneumonocyte injury and inflammatory response induced by MA could associate with the changes of TNF-α and IL-6.

关 键 词：丙烯酸甲酯 小鼠 肺组织 肿瘤坏死因子-Α 白细胞介素-6 

分 类 号：R135.1[医药卫生—劳动卫生] R994.3[医药卫生—公共卫生与预防医学]