雷公藤固态生物转化产物减毒增效作用的实验研究  

More activity and less toxicity of extracts from solid fermented medicinal plant Tripterygium wilfordii

作　　者：马伟光[1] 毕云[2] 黄之镨[1] 钱如贵 李婵娟 浦仕彪 

机构地区：[1]云南中医学院天然药物生物转化研究中心,云南昆明650500 [2]昆明医学院,云南昆明650032 [3]云南省药物研究所,云南昆明650111

出　　处：《中草药》2010年第6期927-930,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30660223);云南省财政厅资助项目;云南省政府改善办学条件专项资金资助项目

摘　　要：目的观察与比较经曲霉菌种和根霉菌种转化后的雷公藤甲醇总提取物的药理活性和毒性反应,为筛选具有保持或提高雷公藤活性、降低毒性的生物转化菌种提供实验依据。方法采用急性和慢性炎症模型、免疫器官质量及细胞免疫试验考察转化产物的药理活性;采用ip给药途径观察急性毒性反应。结果曲霉菌种转化后甲醇总提取物(TW1)的抗炎、免疫抑制作用略有增强,且毒性明显降低;根霉菌种转化总提取物(TW2)毒性有所降低,但抗炎、免疫抑制作用也略有下降。结论曲霉菌种和根霉菌种转化后的甲醇总提取物在抗炎、免疫抑制作用和急性毒性方面发生了一定的变化,减毒增效作用以曲霉菌种较为明显。Objective In order to reduce the toxicity and still maintain the bioactivity of the medicinal plant Tripterygium wilfordii（TW）,the whole plant biotransfomed method was employed.By observing the pharmacological activities and the toxicity of MeOH extracts from the root of T.wilfordii that were biotransformed by the fungi Aspergillums spp.and Rhizopus spp.,the best biotransformed fungi strain has been selected based on the experimental data.Methods Applying the models of acute and chronic inflammation, the weight of immune organ and cell immunity were used to study the pharmacological activities,and ip injection to observe the acute toxicity.Results The anti-inflammation and immunosuppression have been increased slightly and the toxicity decreased significantly after being biotransformed by Aspergillus spp.（TW1）,while the anti-inflammation,immunosuppression,and the toxicity have been declined after being biotransformed by Rhizopus spp.（TW2）.Conclusion Although both selected fungi could change the pharmacological activities and toxicity of the T.wilfordii,Aspergillus spp.is better than Rhizopus spp.

关 键 词：雷公藤 生物转化 曲霉菌 根霉菌 

分 类 号：R285.5[医药卫生—中药学]