基于线粒体细胞色素b基因全序列的海南长臀鮠南渡江种群遗传变异分析  

Genetic variation of cranoglanis bouderius multiradiatus population in Nandujiang River base on mitochondrial cytochrome b sequences

作　　者：乐小亮[1] 赵爽[1] 刘海林[1] 章群[1] 

机构地区：[1]暨南大学水生生物研究所

出　　处：《生态科学》2010年第3期247-250,共4页Ecological Science

基　　金：国家自然科学基金(30770415);留学归国人员科研启动基金(2005)资助项目

摘　　要：测定了海南南渡江15尾海南长臀鮠（Cranoglanis bouderius multiradiatus）的cytb基因全序列1138bp，发现3个可变位点和1个简约信息位点，共检测到4种单倍型。海南长臀鮠Cytb基因具有典型的起始密码子（ATG）和不完全终止密码子（T＊＊），共编码379个氨基酸，密码子第三位点G的含量仅2.9%，而C和A的含量分别为39.1%和38.5%，在第二位点上T的使用频繁高达41.3%，表明cytb基因在密码子碱基的使用具有偏倚性。海南长臀鮠基于Kimura-2-parameter模型的单倍型间遗传距离为0.0009~0.0017，在NJ系统树中没有明显的谱系结构，单倍型多样性（0.600）和核苷酸多样性（0.0006）都较低，表明海南长臀鮠遗传多样性非常贫乏，应及时采取有效措施保护海南南渡江种群。The 1138 bp sequence of complete mitochondrial cytochrome b gene （cytb） of 15 individuals of Cranoglanis bouderius multiradiatus population in Nandujiang River, HaiNan Province were determined. 3 variable sites and 1 parsimony-informative sites were detected in 4 hapoltypes; there were a typical initiation codon （ATG） and incomplete termination codon （T＊＊） in the Cytb gene of Cranoglanis bouderius multiradiatus, and the content of Gs in the third codon position was only accounted for 2.9%, but the content of Cs and As was 39.1 % and 38.5% respectively. The content of Ts in the second codon position was 41.3%, indicating that the usage of 4 bases in different codon positions of cytb gene were biased. Genetic distances based on Kimura 2-parameter model among 4 haplotypes ranged from 0.0009 to 0.0017, and no obvious genealogical cluster were found in the neighbor-Joining tree. The result that haplotype diversity （Hd） and nucleotide diversity（Pi） were 0.600 and 0.0006 respectively in the population suggested that genetic diversity of sequences of cytb gene of Cranoglanis bouderius multiradiatus in the Nandujiang River was very poor, and urgent and effective measures should be taken to conserve the valuable fish.

关 键 词：海南长臀能 细胞色素B 遗传变异 

分 类 号：Q178.532[生物学—水生生物学]