庚型肝炎病毒不同地理株5'端非编码区序列的变异性分析  

Diversity Analysis of 5′ NCR Sequence of Hepatitis G Virus from Different Geographical Regions

作　　者：李刚[1,2,3,4] 姚集鲁[1,2,3,4] 廖家杰 姚春斓[1,2,3,4] 梁英杰 汤文辉[1,2,3,4] 

机构地区：[1]中山医科大学传染病学教研室 [2]香港大学玛丽医院胃肠肝病科 [3]粤港肝炎研究中心 [4]昆明医学院附属第一医院传染科

出　　处：《中山医科大学学报》1999年第1期61-64,共4页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：国家自然科学基金

摘　　要：目的：为明确了解中国不同地区（广东、云南、香港）庚型肝炎病毒（ＨＧＶ）株５′端非编码区（ＮＣＲ）序列的差异。方法：采用逆转录巢式聚合酶链反应（ＲＴＰＣＲ）技术，从广东省２例、云南省１例、香港３例共６例ＨＧＶ感染者血浆中扩增ＨＧＶ５′ＮＣＲｃＤＮＡ片段，为２３８ｂｐ，ＰＣＲ产物纯化后直接经双脱氧链末端终止法测定核苷酸序列。结果：中国ＨＧＶ株间５′ＮＣＲ相应序列的同源性介乎９２９３％～９７９８％，与国外分离株比较，同源性介乎８６３６％～９１９２％。结论：中国ＨＧＶ株间５′ＮＣＲ相应序列的同源性较大，与国外分离株的同源性较小；碱基变异在序列中呈散在分布，部分区段变异较大；ＨＧＶ核酸变异与地域因素有关。Objective: To explore the nucleotide sequence variation of 5′ non coding region (5′ NCR) of hepatitis G virus (HGV) strains from different districts in China (including Guangdong province, Yunnan province, Hongkong). Methods: HGV RNAs derived from the plasmas of two patients with hepatitis non A to E and one patient with hepatocellular carcinoma in Guangdong province, and an intravenous drug user in Yunnan province, and three patients with post trans plantation of bone marrow in Hongkong, totally b patients , were converted to cDNA by reverse transcription and subsequently amplified by polymerase chain reaction using 5′ NCR primers. The products with 238 bp were purified and then directly sequenced. Results: The nucleotide homology of HGV 5′ NCR was 92.93%～97.98% among strains isolated in China, and was 86.36%～91.92% between strains from China and several reported isolates from abroad. Conclusions: The homology was higher among strains isolated from China than that between strains from China and isolates from abroad. Nucleotide differences were distributed throughout the sequence, but striking diversity region was observed. Our results suggest that the nucleotide variation of HGV is associated with geographical factor.

关 键 词：庚型肝炎病毒 聚合酶链反应 碱基序列 变异(遗传学) 

分 类 号：R512.63[医药卫生—内科学] R373.2[医药卫生—临床医学]