鸡胚胎微量血采集和其血红蛋白叶酸盐水平测定及意义  

Significance of collection of chick embryo blood specimen for the determination of hemoglobin folate

作　　者：李勇[1] 陈星[1] 李竹[1] 

机构地区：[1]北京医科大学中国妇婴保健中心

出　　处：《卫生研究》1999年第1期37-39,共3页Journal of Hygiene Research

基　　金：国家"863"计划;国家自然科学基金

摘　　要：建立了１０日龄鸡胚胎微量血采集方法，尿绒膜血管采血量约为１００μｌ／胚胎，制成的纸血片再用微生物法测定其血红蛋白叶酸盐含量（叶酸盐／血红蛋白；μｇ／ｇ）。实验结果表明，滴至在鸡胚胎内壳膜表面的叶酸（ｆｏｌｉｃａｃｉｄ，ＦＡ）和同型半胱氨酸（ｈｏｍｏｃｙｓｔｅｉｎｅ，ＨＣＹ）均能在短期内迅速通过内壳膜和尿绒膜而被吸收入血。滴注５μｇＦＡ后，血红蛋白叶酸盐吸收高峰时间点为６０ｍｉｎ时，剂量为２２．２６μｇ／ｇ。单纯滴注ＨＣＹ（８μｍｏｌ／胚胎）对胚胎血红蛋白叶酸盐水平影响不大（Ｐ＞０．０５）。ＦＡ和ＨＣＹ联合给药６０ｍｉｎ后，胚胎血红蛋白的叶酸盐水平明显下降（Ｐ＜０．００１），呈拮抗作用。此外。A method for embryo blood collection (about 100μl/embryo) from chorioallantoic blood vessels of day 10 White Leghorn chick embryo was developed and the embryonic hemoglobin folate concentrations (folate/hemoglobin, μg/g) were measured by the microbiological assay on microtitre dried blood spots to test the hypothesis whether homocysteine (HCY) would increase in folate depletion during early development stage of embryo. The results showed folic acid (FA) and HCY having been placed in the enlarged air cell could quickly pass through inner shell membrane and chorioallantoic membrane, and enter into blood, on the highly vascularized membrane of chick embryo aged 10 days. After 5 μg FA were placed on the inner shell membrane, there was a striking increase of hemoglobin folate with a peak in 60 min at 22 26 μg/g, followed by a decline. In contrast, 8 μmol of D.L HCY did not result in the obvious increase of embryonic hemoglobin folate during the total transient time course (0～480 min). By 60 min posttreatment the hemoglobin folate concentration for joint group (HCY 8μmol+FA 5μg/embryo) had obviously decreased in comparison with single FA group ( P <0 001). The results supported the above hypothesis mentioned. Moreover, both advantages and disadvantages of this method were evaluated.

关 键 词：胚胎 叶酸 血红蛋白叶酸盐 微量血 采血 

分 类 号：Q592.1[生物学—生物化学]