降解材料隔膜引导性骨再生的实验研究  

Experimental Study on Bone Regeneration Guided by Biodegradable Membrane

作　　者：张永刚[1] 卢世璧[1] 王继芳[1] 张伯勋[1] 解英俊[1] 

机构地区：[1]解放军总医院骨科,北京100853

出　　处：《中华创伤杂志》1999年第1期5-8,共4页Chinese Journal of Trauma

摘　　要：目的观察长管骨降解材料隔膜引导性骨再生现象，探讨其作用机制。方法采用胶原隔膜，制做长管骨引导性骨再生动物模型。术后进行Ｘ线观察；不同时期处死取材，进行组织学观察及碱性成纤维细胞生长因子（ｂＦＧＦ）、转移生长因子－β（ＴＧＦ－β）、骨形态发生蛋白（ＢＭＰ）免疫组化染色，并对３种骨诱导因子的含量进行测量。结果（１）胶原隔膜能够成功引导骨再生，１２周时修复骨缺损。（２）术后１周时，胶原隔膜即在骨缺损处形成密闭的管状腔室，术后６－８周降解，降解后仍有骨痴形成。（３）术后调内实验侧ｂＦＧＦ、ＴＧＦ－β、ＢＭＰ总体含量明显高于对照侧（Ｐ＜０．００１）。结论术后６周内胶原隔膜在骨缺损处形成密闭腔室，将周围组织隔离于骨缺损之外，提供骨再生的空间，并将骨缺损处骨诱导因子浓缩，成功诱导骨组织再生；６－８周后胶原隔膜降解不影响骨再生过程。结果表明，引导性骨再生早期的骨形成具有重要的作用，引导性骨再生启动后，即使８周隔膜降解，仍不影响骨缺损修复。Objective To observe the gtlided bone regeneration (GBR) in long bone,so as to find the role of biodegradable membrane in GBR. Methods New Zealand rabbitswere used to produce GBR models with collag6n membrane. The callus was examinedpostoperatively by x-ray. And the histology and the content of BMP, TGF- β, andbFGF by their immunohistochemical staining at different time were measured. Results 1.The bone defects were repaired successfully by GBR with collagen membrane at 12weeks after operation. 2. The sealed bone chamber was formed by collagen membranetube, which degraded within 6 or & weeks, after that callus was formed. 3. The wholecontent of BMP, TGF- β, and bFGF in the test sides was much more higher than thatof the control sides (P <0.001). Conclusion it indicates that the early callus plays a veryimpohant role in GBR. After GBR was started, the bone defects will be repaired by GBRin spite of degradation of collagrn membrane at 8 weeks.

关 键 词：降解隔膜 胶原膜 骨缺损 骨再生 骨折 

分 类 号：R683.05[医药卫生—骨科学] R318.08[医药卫生—外科学]