硫化氢／胱硫醚-γ-裂解酶系统在肺缺血／再灌注损伤中的作用  

Role of hydrogen sulfide/cystanthionine-γ-splitting enzyme system in rats with lung ischemia/reperfusion injury

作　　者：王兴民 季海锋 王万铁[2] 石璐[2] 贾旭广[2] 

机构地区：[1]浙江省瑞安市中医院,浙江瑞安325200 [2]温州医学院病理生理教研室,浙江温州325053

出　　处：《中国中西医结合急救杂志》2010年第3期155-158,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：浙江省温州市科技计划项目（Y20090326）;浙江省瑞安市科技计划项目（20083075）

摘　　要：目的探讨硫化氢／胱硫醚-γ-裂解酶（H2S／CSE）系统在肺缺血／再灌注损伤（LIRI）中的作用。方法将40只SD大鼠随机均分成假手术组、LIRI组、NaHS组、炔丙基甘氨酸（PPG）组4组。制备在体大鼠单肺原位LIRI模型。NaHS组和PPG组分别于制模前腹腔注射NaHS 28μmol／kg或PPG30mg／kg；假手术组和LIRI组给予生理盐水0．5ml。实验结束后取血，检测血浆H2S浓度、超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）活性和丙二醛（MDA）含量；检测肺组织H2S含量、CSE活性和CSE mRNA表达水平；计算肺系数及肺泡损伤率（IAR）；并观察肺组织病理改变。结果与假手术组比较，LIRI组血浆和肺组织H2S水平、肺组织CSE活性和CSE mRNA表达均降低（均P〈0．01），血浆SOD降低，MDA、MPO升高（均P〈0．01）；肺系数、IAR增高（均P〈0．01）；同时LIRI组肺组织有明显的病理改变。与LIRI组比较，预先给予NaHS组血浆和肺组织H2S水平、肺组织CSE活性和CSE mRNA表达均升高（均P〈0．01），血浆SOD升高，MDA、MPO降低（均P〈0．01），肺系数、IAR降低（均P〈0．01）；同时肺组织病理改变减轻。而预先给予PPG组则相反，可加重LIRI所致的肺损伤（P〈0．05或P〈0．01）。结论H2S／CSE系统功能下调参与LIRI的病理过程，预先给予NaHS对肺组织有保护作用。Objective To investigate the role of hydrogen sulfide/eystanthionine-γ-splitting enzyme （H2S/CSE） system in lung ischemia/reperfusion injury （LIRI）. Methods A total of 40 Sprague-Dawley （SD） rats were divided at random into four groups： sham operation control （sham） group, LIRI group, sodium hydrosulfide （NaHS） group, and propargylglycine （PPG） group. The animal model of single lung isehemia and reperfusion was established in vivo. NaHS （28 μmol/kg） was given in the Naris group and PPG （30 mg/kg）, in the PPG group by intraperitoneal injection before the model was made, while the sham group and LIRI group were given equivalent normal saline 0.5 ml. The blood specimens gathered at the end of experiments were tested for the content of H2S, melondialdehyde （MDA）, the enzyme activities of superoxide dismutase （SOD） and myeloperoxidase （MPO）. The content of H2S, the CSE enzymatic activity and the expression of CSE mRNA in lung tissues were measured. The lung/body weight ratio （LW/BW） and the injured alveoli rate （IAR） were also counted. The histological and ultrastructural changes were observed as well. Results Compared to sham group, the contents of H2S both in blood plasma and lung tissues, the activity of CSE and the expression of CSE mRNA in lung tissues were decreased （all P〈0.01） ;the activity of SOD was decreased and content of MDA, activity of MPO were increased in blood plasma in LIRI group （all P〈0.01）. The values of LW/BW and IAR were significantly higher in LIRI group than those in sham group （both P〈0. 01）. Meanwhile, severe injuries of lung tissues were observed in LIRI group. Compared to LIRI group, the contents of H2S both in blood plasma and lung tissue, the activity of CSE and the expression of CSE mRNA in lung tissues were significantly increased in NaHS group （all P〈0.01） ; the activity of SOD was increased and content of MDA, activity of MPO were decreased in blood plasma in NaHS group （all P〈 0.01）. The values of

关 键 词：硫化氢 胱硫醚-Γ-裂解酶 缺血/再灌注损伤 肺 硫氢化钠 炔丙基甘氨酸 

分 类 号：R256.1[医药卫生—中医内科学] R361.2[医药卫生—中医学]