中药912液对脓毒症大鼠心肌细胞线粒体抗氧化防御体系酶的影响  

Effect of 912 solution on enzymes of myocardial mitochondrial antioxidant defense system in rats with sepsis

作　　者：邵婧[1] 王国兴[1] 金明[1] 张寒钰[1] 杨立沛[1] 谢苗荣[1] 

机构地区：[1]首都医科大学附属北京友谊医院急诊科,北京100050

出　　处：《中国中西医结合急救杂志》2010年第3期163-165,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

摘　　要：目的探讨中药912液对脓毒症大鼠心肌细胞线粒体抗氧化防御体系酶的影响。方法将32只Wistar大鼠按随机数字表法均分为对照组、模型组、912液组、地塞米松组。采用静脉注射脂多糖（LPS）的方法建立大鼠脓毒症模型。912液组于制模前1h灌胃10mg／kg912液6ml；地塞米松组于注射LPS后即刻注射0．5mg／kg地塞米松1ml。各组于制模后6h处死大鼠取心肌组织制备匀浆，用比色法测定心肌细胞线粒体总ATP酶、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）活性及丙二醛（MDA）含量。结果与对照组比较，模型组心肌细胞线粒体总ATP酶、SOD、GSH—Px酶活性均显著降低（均P〈0．01），MDA含量明显升高（P〈0．05）。与模型组比较，912液组线粒体总ATP酶、SOD、GSH—Px活性明显增加（均P〈0．01），MDA含量明显减少（P〈0．05）；地塞米松组仅总ATP酶、SOD活性明显升高（均P〈0．05），GSH—Px活性及MDA含量差异无统计学意义（均P〉0．05）。结论脓毒症时大鼠心肌细胞线粒体出现能量代谢障碍；中药912液可以减轻心肌细胞线粒体的氧化损伤。Objective To investigate the effect of 912 solution on enzymes of myocardial mitochondrial antioxidant defense system in rats with sepsis. Methods Thirty-two Wistar rats were randomly divided into four groups., control, model, 912 and dexamethasone groups. Intravenous injection of lipopolysaccharide （LPS） was applied to establish the rat model of sepsis. One hour before the model establishment, 6 ml of 912 solution （10 mg/kg） was given intragastrically. Immediately after LPS injection, dexamethasone （0.5 mg/kg） 1 ml was injected in the dexamethasone group. Six hours after the model formation, the rats were sacrificed and their cardiac muscles were taken away to prepare homogenates from which myocardial mitochondria were picked up. The kits of colorimetric method were used to determine the activity of total-ATPase, superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and the content of malonaldehyde （MDA） in the myocardial mitochondria. Results Compared with control group, the activity of total-ATPase, SOD, GSH-Px of myocardial mitoehondria were obviously decreased （all P〈0.01）, and MDA was significantly increased in model group （P〈 0.05）. Compared with model group, the activity of total-ATPase, SOD, GSH-Px of mitoehondria were markedly increased （all P % 0.01）, and MDA was obviously reduced in 912 group （P〈0. 05）; in the dexamethasone group, the activity of total-ATPase and SOD were remarkably increased （both P〈0. 05）, while the activity of GSH-Px and the MDA content had no significant differences （both P 〉 0.05）. Conclusion In sepsis, the rat myocardial mitochondria appear impairment in energy metabolism. The Chinese medicine 912 solution can reduce the oxidative injury in myocardial mitochondria.

关 键 词：脓毒症 心肌损伤 线粒体 抗氧化防御体系酶 中药912液 

分 类 号：R285.6[医药卫生—中药学] R256.2[医药卫生—中医学]