Mercury (Hg^(2+)) effect on enzyme activities and hepatopancreas histostructures of juvenile Chinese mitten crab Eriocheir sinensis  

作　　者：赵艳民 王新华 秦延文 郑丙辉 

机构地区：[1]College of Life Sciences, Nankai University [2]River and Coastal Environmental Research Center, Chinese Research Academy of Environmental Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第3期427-434,共8页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Basic Research Program of China (973 Program) (No. 2007CB407306);Basic Scientific Research Operation Cost of State-level Public Welfare Scientific Research Institute of Chinese Research Academy of Environmental Sciences (No. 2007KYYW08)

摘　　要：We studied the effects of mercury (Hg2+) on antioxidant and digestive enzyme activities in terms of LC50 value and on hepatopancreas histostructures of juvenile Chinese mitten crabs Eriocheir sinensis in 40-day exposure to various concentrations of Hg2+ (0, 0.01, 0.05, 0.10, 0.20, and 0.30 mg/L). The results show that the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) significantly increased in the concentrations of 0.01 and 0.05 mg/L, while that of enzyme decreased in 0.10, 0.20 and 0.30 mg/L treatments. Meanwhile, Hg2+ disrupted the histostructures of the hepatopancreas, causing decreases in activities of pepsin, tryptase, amylase, and cellulose, which are synthesized in the hepatopancreas. Moreover, as the Hg2+ concentration increased, the survival rate of the crabs decreased, worst at 56.57% in 0.30 mg/L. Therefore, although crabs are able to tolerate low levels of mercury pollution, high levels lead to cellular injury and tissue damage in hepatopancreas, which then loses some of its vital physiological functions such as absorption, storage, and secretion.We studied the effects of mercury （Hg^2＋） on antioxidant and digestive enzyme activities in terms of LC50 value and on hepatopancreas histostructures of juvenile Chinese mitten crabs Eriocheir sinensis in 40-day exposure to various concentrations of Hg^2＋ （0, 0.01, 0.05, 0.10, 0.20, and 0.30 mg/L）. The results show that the activities of superoxide dismutase （SOD）, glutathione peroxidase （GPX） and catalase （CAT） significantly increased in the concentrations of 0.01 and 0.05 mg/L, while that of enzyme decreased in 0.10, 0.20 and 0.30 mg/L treatments. Meanwhile, Hg^2＋ disrupted the histostructures of the hepatopancreas, causing decreases in activities of pepsin, tryptase, amylase, and cellulose, which are synthesized in the hepatopancreas. Moreover, as the Hg^2＋ concentration increased, the survival rate of the crabs decreased, worst at 56.57% in 0.30 mg/L. Therefore, although crabs are able to tolerate low levels of mercury pollution, high levels lead to cellular injury and tissue damage in hepatopancreas, which then loses some of its vital physiological functions such as absorption, storage, and secretion.

关 键 词：Eriocheir sinensis MERCURY HEPATOPANCREAS antioxidant enzymes histostructure digestive enzymes 

分 类 号：S917.4[农业科学—水产科学]