Tissue culture of three species of Laurencia complex  

作　　者：沈颂东 吴勋建 阎斌伦 贺丽虹 

机构地区：[1]College of Life Sciences, Soochow University(Suzhou) [2]Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第3期514-520,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by the fund from Jiangsu Key Laboratory of Marine Biotechnology (No. 2006HS006);Huaihai Institute of Technology, Lianyungang, China;211-Project Funding of Soochow University (No. 14134908)

摘　　要：To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20℃ and 35 （salinity）; for C. undulatus, 4 500 lx, 20℃ and 30; and for L. tristicha, 4 500 lx, 25℃ and 30.To establish a micropropagation system of three Laurencia complex species (Laurencia okamurai, Laurencia tristicha, and Chondrophycus undulatus) by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20°C and 35 (salinity); for C. undulatus, 4 500 lx, 20°C and 30; and for L. tristicha, 4 500 lx, 25°C and 30.

关 键 词：Laurencia okamurai Laurencia tristicha Chondrophycus undulatus bud regeneration RHIZOID growth factors 

分 类 号：Q943.1[生物学—植物学] TQ465.5[化学工程—制药化工]