BACILLUSSP.BT7木聚糖酶基因的克隆与鉴定  

CLONING AND CHARACTERIZATION OF GENES ENCODING XYLANASE FROM BACILLUS SP. BT 7

作　　者：汤海妹[1] 曾凡亚[1] 张义正[1] 

机构地区：[1]四川联合大学生物工程系

出　　处：《应用与环境生物学报》1999年第1期60-63,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金

摘　　要：报道了从一株有较高半纤维素酶活性的Ｂａｃｉｌｕｓｓｐ．ＢＴ７克隆内切木聚糖酶基因的结果．以大肠杆菌ＸＬ１ｂｌｕｅ为宿主菌，采用水解圈检测法，在含有ＲＢＢ木聚糖（４氧甲基Ｄ葡萄糖苷Ｄ木聚糖Ｒｅｍａｚｏｌ亮蓝Ｒ）的平板上分离到能水解ＲＢＢ木聚糖的阳性克隆３个，对它们进行的限制酶作图和Ｓｏｕｔｈｅｒｎ杂交分析表明。The results of cloning and characterization of the genes encoding endo xylanase from Bacillus sp. BT 7 were reported in this paper. Three positive clones, named pBX1, pBX2 and pBX3, were isolated and they can express in Escherichia coli and form colorless halo on blue background RBB xylan plate. The analyses by restriction mapping and Southern hybridization proved that they were three different endo xylanase genes.

关 键 词：内切木聚糖酶基因 基因克隆 芽胞杆菌 大肠杆菌 

分 类 号：Q78[生物学—分子生物学]