野大麦幼穗原生质体的分离和培养  

PROTOPLAST ISOLATION AND CULTURE OF YOUNG INFLORESCENCES OF WILD BARLEY

作　　者：李彦舫[1] 程肖蕊[1] 张亚兰[1] 庞劲松[1] 

机构地区：[1]解放军农牧大学生物教研室

出　　处：《植物学通报》1999年第1期67-71,共5页Chinese Bulletin of Botany

基　　金：总后军需部资助课题

摘　　要：以野大麦（Ｈｏｒｄｅｕｍｂｒｅｖｉｓｕｂｕｌａｔｕｍ）的幼穗诱导愈伤组织，建立悬浮细胞系，利用胚性悬浮细胞系在不同的酶解条件和不同的酶解液中分离原生质体并进行培养，获得了细胞分裂，形成细胞团。Embryogenic suspension cell line was established from calli which were induced from young inflorescences of wild barley (Hordeum brevisubulatum. After isolated from the suspension cell line under different conditions and enzyme solutions, protoplasts were cultured, cell division and cell cluster were observed.

关 键 词：野大麦 原生质体 细胞分裂 

分 类 号：Q949.714.2[生物学—植物学] S543.903.5[农业科学—作物学]