非水溶性甲胺磷降解酶的检测  

The Detection of A Non water Soluable Methamidophos degrading Enzymatic Activity

作　　者：钞亚鹏[1,2] 赵永芳[1,2] 王银善[1,2] 成汇[1,2] 谢裕敏 

机构地区：[1]中国科学院武汉病毒研究所 [2]武汉大学生命科学学院

出　　处：《中国生物化学与分子生物学报》1999年第1期145-147,共3页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金

摘　　要：甲胺磷农药是一种水溶性广谱、剧毒杀虫剂，化学名称：Ｏ，Ｓ－二甲基胺基硫代磷酸酯．目前，它在我国的生产和使用量已相当可观，并由此造成了严重的生态破坏和环境污染［１］．近几年，作者针对上述问题，结合国内外对甲胺磷代谢和有关酶知识缺乏了解的实际［２］，开展...A non water soluable methamidophos degrading enzyme was extracted from the methylotrophic bacterium WB 1 growing on the minimal medium with methamidophos as sole carbon and nitrogen source in the existence of Tween 20.One percent of Tween 20 when extracted improved the enzyme activity about 4 times;at the same time,three percent of Tween 20 in the determination system could increase the enzyme activity greatly.Different procedures of determination affected the result to a large extent.In addition,moderate NH 4Cl and KCN could also improve the enzyme activity respectively,but the mechanisms were different from each other.

关 键 词：甲胺磷除解酶 检测 制备 

分 类 号：X592[环境科学与工程—环境工程] Q550.3[生物学—生物化学]