2005年版《中国药典》和《欧洲药典》菌落计数培养基比较  

Comparison the Colony Counting Method between the 2005 Version Chinese Pharmacopoeia and the Mainly Overseas Pharmacopoeia

作　　者：由亚宁 陈雪芹 周志云 王小亮[3] 

机构地区：[1]陕西省食品药品检验所,西安710061 [2]西安千禾药业有限责任公司 [3]西安交通大学

出　　处：《中国药事》2010年第6期587-589,共3页Chinese Pharmaceutical Affairs

摘　　要：目的对2005年版《中国药典》和《欧洲药典》菌落计数方法进行比较,对二者所使用培养基的菌落培养结果进行综合分析和评价。方法 77批实验样品,以2005年版《中国药典》菌落计数方法进行比较实验。结果细菌在《欧洲药典》中所使用胰酪胨大豆琼脂培养基(TSA)的生长状况优于《中国药典》中所使用细菌计数营养琼脂培养基(NA);霉菌和酵母菌在《中国药典》中所使用的玫瑰红钠琼脂培养基的生长状况优于《欧洲药典》所使用的沙氏琼脂培养基(TYMC)。结论胰酪胨大豆琼脂培养基是更适合细菌计数的培养基;玫瑰红钠琼脂培养基是更适合霉菌和酵母菌计数的培养基。Objective Compare the colony counting method between the 2005 version Chinese Pharmacoppeia and the mainly overseas pharmacopoeia and carry on the generalized analysis and the appraisal between the two results.Methods Carry out the experiment using 77 batches of samples and compare the results according to the 2005 version Chinese Pharmacopoeia.Results For bacterial,the TSA medium using in overseas pharmacopeia was better than NA medium using in Chinese Pharmacopoeia.For mold and saccharomycetes,the rose pink color sodium agar medium using in Chinese Pharmacopoeia was better than the TYMC culture medium using in overseas pharmacopoeia.Conclusion TSA is more suitable for the colony counting medhod of bacterial and rose pink color sodium agar medium is more suitable for the colony counting method of mold and the saccharomycetes.

关 键 词：培养基 菌落计数方法 菌落计数比较实验 

分 类 号：R921.2[医药卫生—药学] R921.5