星点设计-效应面法优化刺五加叶提取工艺  

Optimum extracting process for leaves of Acanthopanax senticosus(Rupr.et Maxim.) Harms by central composite design and response surface method

作　　者：李攀登[1] 张崇禧[2] 李金玲[1] 陆珞[1] 郑友兰[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]山东大学威海分校海洋学院,威海264209

出　　处：《药物分析杂志》2010年第6期1064-1068,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:星点设计-效应面法优化刺五加叶中紫丁香苷和金丝桃苷的提取工艺。方法:以乙醇体积分数、提取时间、料液比为自变量,紫丁香苷和金丝桃苷提取率为因变量,通过对自变量与因变量的完全二次响应曲面的回归拟合,用岭嵴分析和效应面法选取最佳工艺,并进行预测分析。结果:确定紫丁香苷最佳提取工艺为:体积分数57.56%乙醇水溶液27.75倍量超声提取3次,每次76.28min;金丝桃苷最佳提取工艺为:体积分数58.86%乙醇水溶液29.75倍量超声提取3次,每次59.17min。结论:星点设计-效应面法优选的刺五加叶中紫丁香苷和金丝桃苷的提取工艺,方法简便,精度更高。Objective：To optimize the extracting process of leaves of Acanthopanax senticosus（Rupr.et Maxim.）Harms by central composite design and response surface method.Method：Independent variables were ethanol concentration,extract time,and solvents ratio,dependent variables were extracting rate of syringin and hyperin,composite design and response surface method were used to optimize the extracting process,a two-order polynomial equation was fitted to the data,and resultant model was used to predict the response in the optimal region.Results：Analysis indicated the optimum extraction conditions for syringin were 57.56% aqueous ethanol 76.28 min for once extraction,27.75 fold solvent and 3 times,and that for hyperin were 58.86% aqueous ethanol 59.17 min for once extraction,29.75 fold solvent and 3 times.Conclusion：The optimum precess is simple and more convenient for extracting the leaves of A.senticosus,with an higher precision.

关 键 词：刺五加 星点设计 效应面法 紫丁香苷 金丝桃苷 

分 类 号：R917[医药卫生—药物分析学]