土三七不定芽直接发生与遗传稳定性的RAPD分析  

DIRECT ORGANOGENESIS OF ADVENTITIOUS BUDS OF Sedum aizoon L. AND ANALYSIS OF GENETIC STABILITY IN REGENRATED PLANTLETS BY RAPD

作　　者：岑举人[1] 步怀宇[1] 张来军[1] 王英娟[1] 毕彦博[1] 贾敬芬[1] 

机构地区：[1]西北大学西部资源生物与现代生物技术教育部重点实验室,陕西省生物技术重点实验室,陕西西安710069

出　　处：《核农学报》2010年第3期502-506,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(30970308);陕西省教育厅基金(09JS080;09JK775)

摘　　要：以土三七叶片为外植体,在MS+6-BA2mg/L+NAA1mg/L培养基上直接生成不定芽,经MS+IBA0.2mg/L培养基诱导生根,多次继代培养、移栽,建立了土三七不定芽植株再生体系。用RAPD分析了野生土三七、连续3次继代试管苗和移栽苗的遗传稳定性。结果显示:用选取的20条随机引物共扩增出清晰的88个条带,条带数在2～8条之间,平均4.4条。不同植株扩增获得的条带数目和带型一致,表明在所检测范围内继代培养和移栽未影响土三七DNA序列。所建立的直接再生程序可用于土三七试管苗大量生产。Leaf segments of Sedum Aizoon L. were cultured on MS medium supplemented with 2mg /L 6-BA and 1mg /L NAA. A few of adventitious buds were induced from incision of leaves explants,and regenerated shoots were rooted and subcultured on MS medium with 0. 2mg /L IBA. Random amplified polymorphic DNA （ RAPD） markers were used to assess the genetic stability of regenerated plantlets. The 20 arbitrary primers used in this analysis yielded 88 scoreable bands with an average of 4. 4 bands per primer. These 88 bands were monomorphic with identical bands at DNA level among all shoots from the regenerated plantlets, transplants and control plants of Sedum aizoon L. The results demonstrated that high-efficient direct organogenesis and regeneration system might be used for micropropogation of Sedum aizoo L.

关 键 词：土三七 不定芽直接发生 遗传稳定 RAPD 

分 类 号：S567.239[农业科学—中草药栽培]