检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:赵金梅[1] 李芳[2] 周禾[3] 孙启忠[1] 毕静[2]
机构地区:[1]中国农业科学院草原研究所,内蒙古呼和浩特010010 [2]内蒙古农业大学环境生态学院,内蒙古呼和浩特010018 [3]中国农业大学动物科技学院,北京100193
出 处:《核农学报》2010年第3期507-512,521,共7页Journal of Nuclear Agricultural Sciences
基 金:中央级公益性科研院所基本科研业务费专项资金资助项目(nyhyzx2007-1-01);国家科技支撑计划项目(2008BADB3B03)
摘 要:通过外植体类型、生长素和细胞分裂素浓度等因素对紫花苜蓿愈伤组织诱导、分化影响的试验,建立了高效的紫花苜蓿组织培养体系,即以真叶为外植体,在MS+0.2mg/L2,4-D+0.01mg/LNAA+0.2mg/LKT培养基上进行愈伤组织的诱导,诱导率可达到100%,愈伤组织继代2~3次,然后在MS培养基上分化,分化率可达90%。分化苗转入1/2MS培养基进行生根,形成完整的组培苗。愈伤组织诱导的正交试验表明,2,4-D浓度是影响苜蓿愈伤组织诱导率的主要因素,其次是外植体类型、NAA浓度、6-BA浓度和KT浓度;对苜蓿愈伤褐化率影响较大的因素是外植体类型,其次是KT、2,4-D、6-BA、NAA浓度。外植体类型对愈伤组织诱导率和褐化率的影响均达到极显著水平(P<0.01);生长素(2,4-D、NAA)对苜蓿愈伤组织的诱导作用大于细胞分裂素(6-BA、KT)。分化试验表明,紫花苜蓿愈伤组织分化的主要决定因素是愈伤诱导培养基的激素配比。The high efficiency alfalfa regeneration system was established by studying the influence of explants type, auxins and cytokinins concentration on alfalfa callus induction and differentiation in the experiment. The system was that alfalfa callus were induced on MS + 0. 2mg /L 2,4-D + 0. 01mg /L NAA + 0. 2mg /L KT from leaf; callus was subcultured 2 ~ 3 times and then transferred to MS media for shoot development; and transferred to 1 /2 MS media for roots formation. In the alfalfa culture system,callus induction rate could reach 100% and differentiation rate 90% . The orthogonal experiment of callus induction showed that the major factor on alfalfa callus induction ratio was 2,4-D concentration,the next were explants type,NAA,6-BA and KT concentration. And the factors on alfalfa callus browning rate in the experiment ordinally were explants types,KT,2,4-D,6-BA,NAA. Explants types were significantly effect on callus induction rate and browning rate (P 0. 01). Auxins (2,4-D and NAA) had greater effect on alfalfa callus induction than cytokinins (6-BA and KT). The shoot development experiment showed that the hormone component of callus induction media was the determinant factor of alfalfa callus differentiation.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.222