心肌梗死患者ALOX5AP基因SG13S114T/A和SG13S89G/A多态性研究  

Association between gene polymorphism of ALOX5AP (SG13S114T/A and SG13S89G/A ) and myocardial infarction

作　　者：庄义浩[1] 周炯峰[1] 倪素丹[1] 杨俊芬[1] 吴维青[2] 谢建生[2] 张奕[2] 

机构地区：[1]深圳市第二人民医院心内科,518035 [2]深圳市妇幼保健院

出　　处：《中国心血管杂志》2010年第3期186-189,共4页Chinese Journal of Cardiovascular Medicine

基　　金：深圳市科技计划项目(200702013)

摘　　要：目的探讨中国汉族人群中的ALOX5AP基因SG13S114T／A和SG13S89G／A多态性与心肌梗死的关系。方法应用聚合酶链式反应．限制性片段长度多态性（PCR—RFLP）方法对95例急性心肌梗死（AMI）患者和114名健康对照者外周血提取的DNA样本，进行SG13S114T／A和SG13S89G／A点突变分析，对突变型进行DNA测序验证。结果SG13S114T／A及SG13S89G／A单核苷酸多态位点基因频率在AMI组和对照组与HapMap报道数据差异无统计学意义。基因多态性与心肌梗死发病情况的相关情况：SG13S114A／A（OR=1．264，95％可信区间：0．637—2．510，P=0．45）：SG13S114A／T（OR=1．127，95％可信区间：0．670～1．897，P=0．20）；SG13S114T／T（OR=0．745，95％可信区间：0．424～1．311，P=1．04）。结论无显著证据表明ALOX5AP基因SG13S114T／A和SG13S89G／A多态性与心肌梗死发病风险有密切相关性。Objective To investigate the association between gene polymorphism of ALOX5AP and myocardial infarction in Han people. Methods SNPs SG13S114 and SG13S89 in the ALOX5AP were selected for further genotyping in this case-control cohort [ 95 acute myocardial infarction （AMI） patients; 114 controls ] with the polymerase chain reaction and the restriction fragment length polymorphism. ALOX5AP gene variants between AMI patients and controls were analyzed. Results The frequencies of SNPs SG13Sl14 and SG13S89 in AMI patients and controls were similar to that in the database of HapMap. Association between the ALOX5AP polymorphisms and AMI were showed as follows： SG13S114A/A：OR = 1. 264 （95% CI：0. 637-2. 510;P =0. 45 ） ;SG13S114A/T： OR = 1. 127 （95% CI： 0. 670-1. 897;P =0. 20） ;SG13S114T/T：OR =0. 745 （95% CI：0. 424-1. 311 ;P = 1.04）. Conclusions Our results does not show close relationship between ALOXSAP genetype SG13S114T/A and SG13S89G/A and myocardial infarction.

关 键 词：心肌梗死 基因 多态性 限制性片段长度 

分 类 号：R542.22[医药卫生—心血管疾病]