抗大麦黄矮病毒GPV株系小麦异附加系Z1抗性基因的RAPD标记  

RAPD MARKER OF RESISTANCE GENE TO STRAIN GPV OF BYDV IN WHEAT ADDITION LINE Z1

作　　者：孙晓平[1] 钱幼亭[1] 周广和[1] 

机构地区：[1]中国农业科学院植物保护研究所

出　　处：《植物病理学报》1999年第1期37-40,共4页Acta Phytopathologica Sinica

摘　　要：经生物学方法鉴定，小麦－中间偃麦草异附加系Ｚ１高抗我国特有的大麦黄矮病毒ＧＰＶ株系，用１０４个随机引物对Ｚ１及其亲本中７９０２的基因组进行ＲＡＰＤ分析，发现其中的引物ＯＰＳ－１６能从Ｚ１中扩增１个约１．７Ｋｂ的特异性片段。用引物ＯＰＳ－１６扩增Ｚ１、中７９０２、中间偃麦草、中５，同样也从Ｚ１、中间偃麦草、中５中能稳定地扩增此１．７Ｋｂ片段。对Ｚ１的抗、感病单株的自交后代进行ＲＡＰＤ分析，发现在所测的抗病株的自交后代中能稳定地扩增此１．７Ｋｂ片段，而在感病株的自交后代中则不能扩增。The wheat-Elytrigia intermedia addition line Z1 was identified to be highly resistant to strain GPV of BYDV, and this strain exists only in China. The genomes of resistant lines and susceptible lines were analyzed by RAPD using 104 random primers. It was found that primer OPS16 can amplify a specific 1.7 Kb fragment from genome of Z1.When Z1, Zhong7902, Elytrigia intermedia and Zhong5 were amplified using primer OPS16, this specific 1.7 Kb fragment was also produced in Elytrigia intermedia, Zhong5 and Z1. When selfbred progenies of resistant and susceptible Z1 individuals were amplified using primer OPS16, this specific 1.7 Kb fragment was amplified in progenies of resistant individuals of Z1, but not in progenies of susceptible individuals.

关 键 词：RAPD 大麦黄矮病毒 异附加系 

分 类 号：S435.123[农业科学—农业昆虫与害虫防治]