自然侵染菜豆的花生矮化病毒研究  

IDENTIFICATION AND CHARACTERIZATION OF AN ISOLATE OF PEANUT STUNT VIRUS FROM NATURALLY INFECTED BEANS IN CHINA

作　　者：张宗义[1,2] 陈坤荣[1,2] 许泽永 方小平[1,2] 晏立英 

机构地区：[1]中国农业科学院油料作物研究所 [2]农业部油料作物遗传改良重点开放实验室

出　　处：《植物病理学报》1999年第1期68-72,共5页Acta Phytopathologica Sinica

基　　金：国家自然科学基金;"九五"农业部重点科研项目

摘　　要：对从北京郊区菜豆皱缩花叶病株上获得的一个病毒分离物进行了系统研究。根据该分离物的寄主范围、传播特性、病毒颗粒形态、血清学性质和理化特性等，确定为花生矮化病毒（ＰｅａｎｕｔＳｔｕｎｔＶｉｒｕｓ，ＰＳＶ），并称为ＰＳＶ菜豆分离物，即ＰＳＶＢｅａｎＩｓｏｌａｔｅ，简称ＰＳＶ－Ｂ。这是国内从菜豆上分离ＰＳＶ的首次报道。在测定的５科２８种植物中，ＰＳＶ－Ｂ侵染５科２１种。ＰＳＶ－Ｂ致死温度６０～６５℃，稀释限点１０－４～１０－５，存活期限６ｄ以上。ＰＳＶ－Ｂ能被豆蚜（ＡｐｈｉｓｃｒａｃｉｖｏｒａＫｏｃｈ）以非持久性方式传播。提纯病毒颗粒球形，直径３０ｎｍ。制备的ＰＳＶ－Ｂ抗血清，采用琼脂双扩散法测定效价为１１２８。在血清学关系上，ＰＳＶ－Ｂ与已报道的中国ＰＳＶ－Ｍｉ株系相近，与美国ＰＳＶ－Ｅ株系差异明显。ＰＳＶ－Ｂ外壳蛋白亚基分子量为２５０００道尔顿；含有４个ＲＮＡ片段。Abstract A virus isolated from a diseased bean plant in the Beijing suburb was identified as peanut stunt virus, designated PSV Bean isolate (PSV-B) on the basis of host range, transmission characteristics, in vitro properties, virus particle morphology, serology and physical and chemical properties, which was first reported in China. PSV-B infected 21 of 28 species tested in five families. The thermal inactivation point was 60-65. The dilution end point was 10-4-10-5 and the longevity in vitro was 6 days. Aphis craccivora Koch transmitted the virus in a nonpersistent manner. The virus was successfully purified by modified Franck's method for purification of CMV, virus yield was 19.6 mg/100 g infected tissue. Virus particles were spherical with 30 nm in diameter. An antiserum against PSV-B was produced with titers of 1128 in gel diffusion tests. Serologically PSV-B was closely related to PSV-Mi, distantly related to PSV-E. The molecular weight of PSV-B coat protein is about 25 000 D.PSV-B has four kinds of strand RNA.

关 键 词：菜豆 皱缩花叶 花生矮化病毒 鉴定 

分 类 号：S436.43[农业科学—农业昆虫与害虫防治]